Stabilization of glutathione redox dynamics and CYP2E1 by green synthesized Moringa oleifera-mediated zinc oxide nanoparticles against acrylamide induced hepatotoxicity in rat model: Morphometric and molecular perspectives

被引：16

作者：

Mahfouz, Hala ^{[1
]}

Dahran, Naief ^{[2
]}

Mohamed, Amany Abdel-Rahman ^{[3
]}

Abd El-Hakim, Yasmina M. ^{[3
]}

Metwally, Mohamed M. M. ^{[4
]}

Alqahtani, Leena S. ^{[5
]}

Abdelmawlla, Hassan Abdelraheem ^{[6
,7
]}

Wahab, Hazim A. ^{[8
]}

Shamlan, Ghalia ^{[9
]}

Nassan, Mohamed A. ^{[10
]}

Gaber, Rasha A. ^{[11
]}

机构：

[1] Kafrelsheikh Univ, Fac Med, Dept Med Biochem & Mol Biol, Kafrelsheikh, Egypt

[2] Univ Jeddah, Fac Med, Dept Anat, Jeddah, Saudi Arabia

[3] Zagazig Univ, Dept Forens Med & Toxicol, Zagazig, Egypt

[4] Zagazig Univ, Fac Vet Med, Dept Pathol, Zagazig 44511, Egypt

[5] Univ Jeddah, Coll Sci, Dept Biochem, Jeddah 23445, Saudi Arabia

[6] Jouf Univ, Coll Med, Dept Anat, Sakaka, Saudi Arabia

[7] Beni Suef Univ, Coll Med, Anat & Embryol Dept, Bani Suwayf, Egypt

[8] Menofiya Univ, Fac Med, Histol Dept, Shibin Al Kawm, Egypt

[9] King Saud Univ, Coll Food & Agr Sci, Dept Food Sci & Nutr, Riyadh 11362, Saudi Arabia

[10] Taif Univ, Turabah Univ Coll, Dept Clin Lab Sci, POB 11099, Taif 21944, Saudi Arabia

[11] Tanta Univ, Fac Med, Med Biochem Dept, Tanta, Egypt

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2023年 / 176卷

关键词：

Green synthesis; Zinc oxide nanoparticles; Moringa oleifera; glutathione; Caspase-3; Hepatotoxicity; INDUCED OXIDATIVE STRESS; TNF-INDUCED APOPTOSIS; GENE-EXPRESSION; IN-VITRO; EXPOSURE; NEUROTOXICITY; GENOTOXICITY; GLYCIDAMIDE; EXTRACT; SERUM;

D O I：

10.1016/j.fct.2023.113744

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The terrible reality is that acrylamide (AA) is a common food contaminant found in a wide variety of commonly consumed foods. This research involves the advancement of a more dependable technique for the bio-fabrication of zinc oxide nanoparticles (ZNPs) through the green method using Moringa Oleifera extract (MO-ZNPs) as an efficient chelating agent for acrylamide (AA). The effects of AA on glutathione redox dynamics, liver function, lipid profile, and zinc residues in Sprague Dawley rats are investigated. Finally, the microarchitecture and immunohistochemical staining of Caspase-3 and CYP2E1 were determined in the liver tissue of rats. Four separate groups, including control, MO-ZNPs (10 mg/kg b. wt), AA (20 mg/kg b. wt), and AA + MO-ZNPs for 60 days. The results revealed a suppressed activity of glutathione redox enzymes (GSH, GPX,and GSR) on both molecular and biochemical levels. Also, AA caused elevated liver enzymes, hepatosomatic index, and immu-nohistochemical staining of caspase-3 and CYP2E1 expression. MO-ZNPs co-treatment, on the other hand, sta-bilized glutathione-related enzyme gene expression, normalized hepatocellular enzyme levels, and restored hepatic tissue microarchitectures. It could be assumed that MO-ZNPs is a promising hepatoprotective molecule for alleviating AA-induced hepatotoxicity. We witnessed changes in glutathione redox dynamics to be restor-ative. Glutathione and cytochrome P450 2E1 play crucial roles in AA detoxification, so maintaining a healthy glutathione redox cycle is necessary for disposing of AA toxicity.

引用

页数：13