Combining atomic force microscopy with complementary techniques for multidimensional single-cell analysis

被引:7
|
作者
Li, Mi [1 ,2 ,3 ]
机构
[1] Chinese Acad Sci, Shenyang Inst Automat, State Key Lab Robot, Shenyang 110016, Peoples R China
[2] Chinese Acad Sci, Inst Robot & Intelligent Mfg, Shenyang, Peoples R China
[3] Univ Chinese Acad Sci, Beijing, Peoples R China
基金
中国国家自然科学基金;
关键词
atomic force microscopy; fluidic force microscopy; infrared spectroscopy; scanning near-field ultrasound holography; single-cell analysis; tip-enhanced Raman scattering; traction force microscopy; ENHANCED RAMAN-SPECTROSCOPY; LIVING CELLS; SUPERRESOLUTION MICROSCOPY; LIGHT-MICROSCOPY; HETEROGENEITY; AFM; CANCER; MECHANICS; STIFFNESS; PROTEINS;
D O I
10.1111/jmi.13183
中图分类号
TH742 [显微镜];
学科分类号
摘要
The advent of atomic force microscopy (AFM) provides an amazing instrument for characterising the structures and properties of living biological systems under aqueous conditions with unprecedented spatiotemporal resolution. In addition to its own unique capabilities for applications in life sciences, AFM is highly compatible and has been widely integrated with various complementary techniques to simultaneously sense the multidimensional (biological, chemical and physical) properties of biological systems, offering novel possibilities for comprehensively revealing the underlying mechanisms guiding life activities particularly in the studies of single cells. Herein, typical combinations of AFM and complementary techniques (including optical microscopy, ultrasound, infrared spectroscopy, Raman spectroscopy, fluidic force microscopy and traction force microscopy) and their applications in single-cell analysis are reviewed. The future perspectives are also provided.
引用
收藏
页码:69 / 96
页数:28
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