Optimization Growth Conditions of Fusarium pseudonygamai for Myco-Synthesized Gold and Silver Nanoparticles Using Response Surface Methodology

Metallic nanoparticles (Au and Ag) were synthesized through simple, rapid, eco-friendly and an economical method with a new cell-free filtrate Fusarium pseudonygamai TB-13 cell as both reducing and stabilizing. The desired nanoparticles (Ag and Au) were optimized using response surface methodology (RSM) to reach the maximum yield of NPs. To attain the ideal reaction state, the central composite face design and the efficient variables in the environmentally friendly synthesis reaction were utilized. Temperature, pH, and precursor concentrations were determined to be the most useful variables for producing Au and Ag nanomaterials. An acceptable foot was employed in the model's realization. The various elements were optimized using surface plots, contour plots, pareto charts,, analysis of variance (ANOVA), normal probability-plots, interaction plots, including effect-plots, and surface plots. A significance criterion of 5% was applied to each and every element. Some of the possible relationships between these variables also had an impact on the production process. The suggested predictive model provided a very good match to the data obtained from experiments. In comparison to conventional optimization methodologies, the study's findings examined whether or not more biofabrication was achievable under ideal fermentation-conditions for the production of Au and Ag nanoparticles.