Characterization and antioxidant activities of polysaccharides from Hippophae rhamnoides L

被引:8
作者
Bao, Xiaowei [1 ]
Wang, Wei [1 ]
Song, Suqing [2 ]
Wei, Chenye [1 ]
Li, Yixin [1 ]
Sun, Jiali [1 ]
Jiang, Junfeng [1 ]
机构
[1] Xinjiang Agr Univ, Coll Food Sci & Pharm, Urumqi 830052, Peoples R China
[2] Xinjiang Acad Agr Sci, Inst Appl Microbiol, Xinjiang Key Lab Special Environm Microbiol, Urumqi 830091, Peoples R China
基金
中国国家自然科学基金;
关键词
Hippophae rhamnoides L. polysaccharide characterization antioxidant activity; IMMUNOMODULATORY ACTIVITIES; PURIFICATION; OPTIMIZATION; EXTRACTION; FRUITS;
D O I
10.1007/s11694-023-02193-z
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
In this paper, the optimal extraction conditions of crude polysaccharide from Hippophae rhamnoides L. were gained by response surface methodology (RSM) according to the principle of Box-Behnken design (BBD), and three purified fractions (PBP-1, PBP-2 and PBP-3) were isolated by multiple column chromatography. PBP-1 mainly contained arabinose, xylose, mannose, glucose and galactose in a molar ratio of 1.18: 1: 2.20: 32.17: 1.45. PBP-2 mainly contained xylose, mannose, glucose and galactose in a molar ratio of 1: 0.28: 1.02: 0.2. PBP-3 mainly contained xylose, glucose and galactose in a molar ratio of 1: 2.15: 0.28, and the average molecular weights were 26.5, 5.6 and 4.9 kDa, respectively. Furthermore, the CPBP and PBP-3 exhibited the higher effective scavenging activities against DPPH, ABTS and reducing power, and the CPBP indicated the highest protective effect against H2O2-induced oxidative injury in LO2 cells. These findings could provide useful information for the utilization and development of the resources of Hippophae rhamnoides L.
引用
收藏
页码:688 / 697
页数:10
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