Micropipette aspiration as a tool for single-particle X-ray imaging and diffraction

被引:0
|
作者
Bruns, Hendrik [1 ]
Hoeppe, Hannes [1 ]
Bellec, Ewen [2 ]
Leake, Steven [2 ]
Osterhoff, Markus [1 ]
Salditt, Tim [1 ]
机构
[1] Georg August Univ Gottingen, Inst Rontgenphys, Friedrich Hund Pl 1, D-37077 Gottingen, Germany
[2] ESRF European Synchrotron, Xray Nanoprobe Grp, 71 Ave Martyrs, F-38000 Grenoble, France
关键词
micropipette aspiration; holographic X-ray imaging; single-particle diffraction; sample delivery of biophysical samples; SCATTERING; VESICLES;
D O I
10.1107/S1600577523003685
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
A sample environment and manipulation tool is presented for single-particle X-ray experiments in an aqueous environment. The system is based on a single water droplet, positioned on a substrate that is structured by a hydrophobic and hydrophilic pattern to stabilize the droplet position. The substrate can support several droplets at a time. Evaporation is prevented by covering the droplet by a thin film of mineral oil. In this windowless fluid which minimizes background signal, single particles can be probed and manipulated by micropipettes, which can easily be inserted and steered in the droplet. Holographic X-ray imaging is shown to be well suited to observe and monitor the pipettes, as well as the droplet surface and the particles. Aspiration and force generation are also enabled based on an application of controlled pressure differences. Experimental challenges are addressed and first results are presented, obtained at two different undulator endstations with nano-focused beams. Finally, the sample environment is discussed in view of future coherent imaging and diffraction experiments with synchrotron radiation and single X-ray free-electron laser pulses.
引用
收藏
页码:788 / 795
页数:8
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