Cross-sectional study of hepatitis E virus (HEV) circulation in Italian pig farms

被引:4
|
作者
Ianiro, Giovanni [1 ]
Pavoni, Enrico [2 ]
Aprea, Giuseppe [3 ]
Romantini, Romina [3 ]
Alborali, Giovanni Loris [2 ]
D'Angelantonio, Daniela [3 ]
Garofolo, Giuliano [3 ]
Scattolini, Silvia [3 ]
De Sabato, Luca [1 ]
Magistrali, Chiara Francesca [4 ]
Burow, Elke [5 ]
Ostanello, Fabio [6 ]
Smith, Richard Piers [7 ]
Di Bartolo, Ilaria [1 ]
机构
[1] Ist Super Sanita, Dept Food Safety Nutr & Vet Publ Hlth, Rome, Italy
[2] Ist Zooprofilatt Sperimentale Lombardia & Emilia, Brescia, Italy
[3] Ist Zooprofilatt Sperimentale Abruzzo & Molise Gi, Teramo, Italy
[4] Ist Zooprofilatt Sperimentale Umbria & Marche Tog, Perugia, Italy
[5] German Fed Inst Risk Assessment, Dept Biol Safety, Berlin, Germany
[6] Univ Bologna, Dept Vet Med Sci, Ozzano Dellemilia, Italy
[7] Anim & Plant Hlth Agcy, Dept Epidemiol Sci, Weybridge, Surrey, England
关键词
hepatitis E virus; HEV; zoonoses; pig; feces; farm; fattening; surveillance; MOLECULAR CHARACTERIZATION; GENOTYPE; 4; PCR ASSAY; SWINE; PREVALENCE; INFECTION; TIME; RISK; SLAUGHTER;
D O I
10.3389/fvets.2023.1136225
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Foodborne transmission is considered the main way of spreading zoonotic hepatitis E virus (HEV) infection in Europe. In recent years, the human cases of hepatitis E in subjects without history of travel in endemic areas have raised, suggesting that domestic HEV transmission is increasing. Pork products with or without liver, are often indicated as the source of many human foodborne HEV cases as well as small outbreaks. Pigs are recognized as the main reservoir of the zoonotic HEV-3 genotype, the most frequently detected in human cases in the EU. In the absence of a harmonized surveillance of HEV circulation, data on prevalence are heterogeneous but confirm a widespread circulation of HEV-3 in pig herds across EU. HEV-3 can pass through the food chain from farm to fork when infected animals are slaughtered. In Italy, several studies reported the circulation of HEV-3 in pig farms, but results are heterogeneous due to different methodologies applied. In the present study, we performed a survey over 51 pig herds belonging to three main types of farms: breeding, fattening and farrow-to-finish. HEV-RNA was analyzed by broad range Real-time RT-PCR on 20 samples for each farm, obtained by pooling together feces from 10 individuals. Overall, HEV RNA was confirmed on 150 fecal pooled samples out of 1,032 (14.5%). At least one positive pooled sample was detected from 18 farms out of 51 tested (35.3%). By lowering the number of infected pigs at primary production, the risk of HEV-3 entering into the food chain can be reduced. Hence, information on HEV circulation in herds is highly relevant for choosing preventive measures and deserves development of a monitoring program and further investigations.
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页数:8
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