Effect of Salt Stress on the Activity, Expression, and Promoter Methylation of Succinate Dehydrogenase and Succinic Semialdehyde Dehydrogenase in Maize (Zea mays L.) Leaves

被引:7
|
作者
Fedorin, Dmitry N. [1 ]
Eprintsev, Alexander T. [1 ]
Caro, Orlando J. Florez [1 ]
Igamberdiev, Abir U. [2 ]
机构
[1] Voronezh State Univ, Dept Biochem & Cell Physiol, Voronezh 394018, Russia
[2] Mem Univ Newfoundland, Dept Biol, St John, NL A1C 5S7, Canada
来源
PLANTS-BASEL | 2023年 / 12卷 / 01期
基金
俄罗斯科学基金会;
关键词
GABA shunt; NaCl; promoter methylation; salt stress; succinate dehydrogenase; succinic semialdehyde dehydrogenase; Zea mays L; GAMMA-AMINOBUTYRIC-ACID; ALTERNATIVE OXIDASE AOX; GENE-EXPRESSION; RESPIRATION; METABOLISM; ACCUMULATION; MITOCHONDRIA; TOLERANCE; MECHANISM; RESPONSES;
D O I
10.3390/plants12010068
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The effect of salt stress on the expression of genes, the methylation of their promoters, and the enzymatic activity of succinate dehydrogenase (SDH) and succinic semialdehyde dehydrogenase (SSADH) was investigated in maize (Zea mays L.). The incubation of maize seedlings in a 150 mM NaCl solution for 24 h led to a several-fold increase in the activity of SSADH that peaked at 6 h of NaCl treatment, which was preceded by an increase in the Ssadh1 gene expression and a decrease in its promoter methylation observed at 3 h of salt stress. The increase in SDH activity and succinate oxidation by mitochondria was slower, developing by 24 h of NaCl treatment, which corresponded to the increase in expression of the genes Sdh1-2 and Sdh2-3 encoding SDH catalytic subunits and of the gene Sdh3-1 encoding the anchoring SDH subunit. The increase in the Sdh2-3 expression was accompanied by the decrease in promoter methylation. It is concluded that salt stress results in the rapid increase in succinate production via SSADH operating in the GABA shunt, which leads to the activation of SDH, the process partially regulated via epigenetic mechanisms. The role of succinate metabolism under the conditions of salt stress is discussed.
引用
收藏
页数:11
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