BM-MSCs alleviate diabetic nephropathy in male rats by regulating ER stress, oxidative stress, inflammation, and apoptotic pathways

被引：5

作者：

Khamis, Tarek ^{[1
,2
]}

Abdelkhalek, Adel ^{[3
]}

Abdellatif, Hussein ^{[4
,5
]}

Dwidar, Nourelden ^{[3
]}

Said, Ahmed ^{[3
]}

Ahmed, Rama ^{[3
]}

Wagdy, Kerolos ^{[3
]}

Elgarhy, Rowina ^{[3
]}

Eltahan, Rawan ^{[3
]}

Mohamed, Hisham ^{[3
]}

Amer, Eman Said ^{[3
]}

Hanna, Maria ^{[3
]}

Ragab, Tarek ^{[3
]}

Kishk, Abdallah ^{[3
]}

Wael, Judy ^{[3
]}

Sarhan, Eyad ^{[3
]}

Saweres, Linda ^{[3
]}

Reda, Mohamed ^{[3
]}

Elkomy, Sara ^{[3
]}

Mohamed, Abdalah ^{[3
]}

Samy, Abdullah ^{[3
]}

Khafaga, Ateya ^{[3
]}

Shaker, Youliana ^{[3
]}

Yehia, Hamdy ^{[3
]}

Alanazi, Asma ^{[6
,7
]}

Alassiri, Mohammed ^{[7
,8
,9
]}

Tirziu, Emil ^{[10
]}

Bucur, Iulia Maria ^{[10
]}

Arisha, Ahmed Hamed ^{[11
,12
]}

机构：

[1] Zagazig Univ, Fac Vet Med, Dept Pharmacol, Zagazig, Egypt

[2] Zagazig Univ, Fac Vet Med, Lab Biotechnol, Zagazig, Egypt

[3] Badr Univ Cairo, Fac Vet Med, Badr, Egypt

[4] Sultan Qaboos Univ, Coll Med & Hlth Sci, Dept Human & Clin Anat, Muscat, Oman

[5] Mansoura Univ, Fac Med, Anat & Embryol Dept, Mansoura, Egypt

[6] King Saud Bin Abdulaziz Univ Hlth Sci KSAU HS, Coll Med, Riyadh, Saudi Arabia

[7] King Abdullah Int Med Res Ctr, Riyadh, Saudi Arabia

[8] King Saud Bin Abdulaziz Univ Hlth Sci KSAU HS, Coll Sci & Hlth Profess, Dept Basic Sci, Riyadh, Saudi Arabia

[9] Minist Natl Guard Hlth Affairs, King Abdulaziz Med City KAMC, Dept Pathol & Lab Med, Riyadh, Saudi Arabia

[10] Univ Life Sci King Mihai I Timisoara ULST, Fac Vet Med, Dept Anim Prod & Vet Publ Hlth, Timisoara, Romania

[11] Badr Univ Cairo, Fac Vet Med, Dept Anim Physiol & Biochem, Badr, Egypt

[12] Zagazig Univ, Fac Vet Med, Dept Physiol, Lab Biotechnol, Zagazig, Egypt

来源：

FRONTIERS IN PHARMACOLOGY | 2023年 / 14卷

关键词：

diabetic nephropathy; mesenchymal stem cells; bone marrow-derived mesenchymal stem cells; diabetes; apoptosis; ER stress; inflammation; intermediate filament proteins; MESENCHYMAL STEM-CELLS; ENDOPLASMIC-RETICULUM STRESS; TUBULAR EPITHELIAL-CELLS; RECENT PROGRESS; KIDNEY-DISEASE; UP-REGULATION; BETA-CELLS; PROTEIN; INJURY; NESTIN;

D O I：

10.3389/fphar.2023.1265230

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Introduction: Diabetic nephropathy (DN), a chronic kidney disease, is a major cause of end-stage kidney disease worldwide. Mesenchymal stem cells (MSCs) have become a promising option to mitigate several diabetic complications.Methods: In this study, we evaluated the therapeutic potential of bone marrow-derived mesenchymal stem cells (BM-MSCs) in a rat model of STZ-induced DN. After the confirmation of diabetes, rats were treated with BM-MSCs and sacrificed at week 12 after treatment.Results: Our results showed that STZ-induced DN rats had extensive histopathological changes, significant upregulation in mRNA expression of renal apoptotic markers, ER stress markers, inflammatory markers, fibronectin, and intermediate filament proteins, and reduction of positive immunostaining of PCNA and elevated P53 in kidney tissue compared to the control group. BM-MSC therapy significantly improved renal histopathological changes, reduced renal apoptosis, ER stress, inflammation, and intermediate filament proteins, as well as increased positive immunostaining of PCNA and reduced P53 in renal tissue compared to the STZ-induced DN group.Conclusion: In conclusion, our study indicates that BM-MSCs may have therapeutic potential for the treatment of DN and provide important insights into their potential use as a novel therapeutic approach for DN.

引用

页数：14

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