Preparation of 3D nano silver trees/sea urchin-like gold and SERS detection of uric acid

In this paper, 3D nano-silver trees/sea urchin-like gold Surface Enhanced Raman Spectroscopy (SERS) substrates were prepared by chemical reduction method, characterized by SEM, EDS, UV-Vis and XRD et.al, and the preparation process was optimized using Rhodamine B (RB) as the Raman signal molecule. The SERS characteristics of Uric Acid on this substrate were investigated and the results showed that the SERS substrate had a Raman enhancement factor of 1.9 x 10(7) for RB, and also had a significant Raman enhancement effect on Uric Acid. The SERS intensity (I-SERS) at 1400 cm(-1) showed a good linear relationship with the logarithm value of uric acid concentration (log C) between the range of 5 x 10(-4) M to 1 x 10(-7) M. The linear fitting equation was I-SERS = 890 (log C) + 8066 (R-2 = 0.983), and the LOD = 2.8 x 10(-)8 M. The spiked test was performed in human serum with recoveries ranging from 82.86% to 125.13% and RSD (n = 3) < 3.5%.