Proteomic and phosphoproteomic characterisation of primary mouse embryonic fibroblasts

被引：1

作者：

Chen, Yanfang ^{[1
,2
]}

Roselli, Severine ^{[1
,2
]}

Panicker, Nikita ^{[3
,4
]}

Brzozowski, Joshua S. ^{[1
,2
]}

Skerrett-Byrne, David A. ^{[5
,6
]}

Murray, Heather C. ^{[1
,2
,7
]}

Verrills, Nicole M. ^{[1
,2
,7
]}

机构：

[1] Univ Newcastle, Hunter Med Res Inst, Coll Hlth Med & Wellbeing, Sch Biomed Sci & Pharm, Callaghan, NSW, Australia

[2] Precis Med Program, Callaghan, NSW, Australia

[3] Univ Newcastle, Hunter Med Res Inst, Coll Hlth Med & Wellbeing, Sch Biomed Sci & Pharm, Callaghan, NSW, Australia

[4] Canc Detect & Therapies Program, Callaghan, NSW, Australia

[5] Univ Newcastle, Hunter Med Res Inst, Prior Res Ctr Reprod Sci, Callaghan, NSW, Australia

[6] Infertil & Reprod Res Program, Callaghan, NSW, Australia

[7] Univ Newcastle, Level 3,Life Sci Bldg, Callaghan, NSW 2308, Australia

来源：

PROTEOMICS | 2024年 / 24卷 / 07期

关键词：

cancer; ECM; fibroblasts; phosphoproteomics; proteomics; KINASE; PROLIFERATION; INHIBITION; ACTIVATION; MIGRATION; REVEALS;

D O I：

10.1002/pmic.202300267

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fibroblasts are the most common cell type in stroma and function in the support and repair of most tissues. Mouse embryonic fibroblasts (MEFs) are amenable to isolation and rapid growth in culture. MEFs are therefore widely used as a standard model for functional characterisation of gene knockouts, and can also be used in co-cultures, commonly to support embryonic stem cell cultures. To facilitate their use as a research tool, we have performed a comprehensive proteomic and phosphoproteomic characterisation of wild-type primary MEFs from C57BL/6 mice. EIF2/4 and MTOR signalling pathways were abundant in both the proteome and phosphoproteome, along with extracellular matrix (ECM) and cytoskeleton associated pathways. Consistent with this, kinase enrichment analysis identified activation of P38A, P90RSK, P70S6K, and MTOR. Cell surface markers and matrisome proteins were also annotated. Data are available via ProteomeXchange with identifier PXD043244. This provides a comprehensive catalogue of the wild-type MEF proteome and phosphoproteome which can be utilised by the field to guide future work.

引用

页数：8

共 43 条

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