Role of Uric Acid in Vascular Remodeling: Cytoskeleton Changes and Migration in VSMCs

被引：9

作者：

Russo, Elisa ^{[1
]}

Bertolotto, Maria ^{[2
]}

Zanetti, Valentina ^{[3
]}

Picciotto, Daniela ^{[3
]}

Esposito, Pasquale ^{[2
,3
]}

Carbone, Federico ^{[2
,3
]}

Montecucco, Fabrizio ^{[2
,3
]}

Pontremoli, Roberto ^{[2
,3
]}

Garibotto, Giacomo ^{[2
]}

Viazzi, Francesca ^{[2
,3
]}

Verzola, Daniela ^{[2
]}

机构：

[1] San Luca Hosp, Nephrol & Dialysis Unit, I-55100 Lucca, Italy

[2] Univ Genoa, Dept Internal Med, I-16132 Genoa, Italy

[3] IRCCS Osped Policlin San Martino, I-16132 Genoa, Italy

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2023年 / 24卷 / 03期

关键词：

uric acid; vascular smooth muscle cell; migration; phenotypic transition; vascular remodeling; SMOOTH-MUSCLE-CELLS; NEOINTIMAL HYPERPLASIA; ARTERIAL STIFFNESS; OXIDATIVE STRESS; PROLIFERATION; HYPERURICEMIA; ACTIVATION; SYSTEM;

D O I：

10.3390/ijms24032960

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mechanisms by which hyperuricemia induces vascular dysfunction and contributes to cardiovascular disease are still debated. Phenotypic transition is a property of vascular smooth muscle cells (VSMCs) involved in organ damage. The aim of this study was to investigate the effects of uric acid (UA) on changes in the VSMC cytoskeleton, cell migration and the signals involved in these processes. MOVAS, a mouse VSMC line, was incubated with 6, 9 and 12 mg/dL of UA, angiotensin receptor blockers (ARBs), proteasome and MEK-inhibitors. Migration property was assessed in a micro-chemotaxis chamber and by phalloidin staining. Changes in cytoskeleton proteins (Smoothelin B (SMTB), alpha-Smooth Muscle Actin (alpha SMA), Smooth Muscle 22 Alpha (SM22 alpha)), Atrogin-1 and MAPK activation were determined by Western blot, immunostaining and quantitative reverse transcription PCR. UA exposition modified SMT, alpha SMA and SM22 alpha levels (p < 0.05) and significantly upregulated Atrogin-1 and MAPK activation. UA-treated VSMCs showed an increased migratory rate as compared to control cells (p < 0.001) and a re-arrangement of F-actin. Probenecid, proteasome inhibition and ARBs prevented the development of dysfunctional VSMC. This study shows, for the first time, that UA-induced cytoskeleton changes determine an increase in VSMC migratory rate, suggesting UA as a key player in vascular remodeling.

引用

页数：16