Fabrication and evaluating the potentials of minocycline hydrochloride-loaded nanofibrous membranes in periodontal guided tissue regeneration: In vivo release and biocompatibility analysis

被引：5

作者：

Ezzati, Alireza ^{[1
]}

Nokhodchi, Ali ^{[2
]}

Saeedi, Morteza ^{[3
]}

Ziaei, Narges ^{[4
]}

Mirzaeei, Shahla ^{[5
,6
]}

机构：

[1] Kermanshah Univ Med Sci, Sch Pharm, Student Res Comm, Kermanshah, Iran

[2] Univ Sussex, Sch Life Sci, Pharmaceut Res Lab, Brighton, England

[3] Rahesh Daru Novine, Pharmaceut Sci Res Ctr, Kermanshah, Iran

[4] Kermanshah Univ Med Sci, Dent Sch, Dept Periodont, Kermanshah, Iran

[5] Kermanshah Univ Med Sci, Hlth Technol Inst, Nano Drug Delivery Res Ctr, Kermanshah, Iran

[6] Kermanshah Univ Med Sci, Hlth Inst, Pharmaceut Sci Res Ctr, Kermanshah, Iran

来源：

JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY | 2023年 / 88卷

关键词：

Electrospinning; Guided tissue regeneration; Membrane; Minocycline hydrochloride; Nanofiber; Periodontitis; ELECTROSPUN NANOFIBERS; IMPLANTS;

D O I：

10.1016/j.jddst.2023.104919

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Guided tissue regeneration (GTR) membranes are space-maintaining devices that can accelerate the healing process of bone and periodontal tissues in periodontitis. These devices possess a high potential to act as controlled-release drug carriers, along with a regenerative effect. The present study aimed to develop minocycline hydrochloride (MINO)-loaded nanofibers as GTR membranes for the enhanced management of periodontitis.Two nanofibrous membranes were independently developed using polyvinyl alcohol (PVA)-collagen and polycaprolactone (PCL) polymeric blends with mixed and sandwich structures. The formulations underwent physiochemical characterization, and an in vitro release study was conducted to observe the manner of drug release. The antimicrobial efficacy against common pathogens involved in periodontitis was evaluated, and in vitro cytotoxicity was assessed. Additionally, the in vivo release profile and biocompatibility were evaluated following subcutaneous implantation of the formulations in rats. High-strength, flexible, and porous nanofibrous GTR membranes with a mean diameter in the submicron range were obtained. The in vitro release study demonstrated a controlled release of the drug within 5 days for both formulations, following a KorsemeyerPeppas kinetic model. In vitro cytotoxicity tests confirmed the safety of the formulations, and suitable antimicrobial efficacy against common pathogens of periodontitis was observed. The in vivo study indicated a prolonged release of minocycline for 15 days, and the pathological investigation did not reveal significant signs of inflammation or tissue damage. The developed GTR membranes exhibited appropriate characteristics to be utilized as GTR membranes, demonstrating the potential for controlled release of MINO and facilitating an accelerated therapeutic response in the treatment of periodontitis.

引用

页数：12

共 46 条

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