Production of Tissue-Engineered Skin Substitutes for Clinical Applications: Elimination of Serum

被引:2
作者
Doucet, Emilie J. [1 ,2 ,3 ]
Cortez Ghio, Sergio [1 ,2 ,3 ]
Barbier, Martin A. [1 ,2 ,3 ]
Savard, Etienne [1 ,2 ,3 ]
Magne, Brice [1 ,2 ,3 ]
Safoine, Meryem [1 ,2 ,3 ]
Larouche, Danielle [1 ,2 ,3 ]
Fradette, Julie [1 ,2 ,3 ]
Germain, Lucie [1 ,2 ,3 ]
机构
[1] Univ Lavals, Tissue Engn Lab LOEX, Res Ctr, Quebec City, PQ G1V 0A6, Canada
[2] Univ Laval, Fac Med, Dept Surg, Quebec City, PQ G1V 0A6, Canada
[3] Univ Laval, Regenerat Med Div, CHU Quebec, Res Ctr, Quebec City, PQ G1V 0A6, Canada
基金
加拿大健康研究院;
关键词
cell culture; tissue engineering; defined medium; stem cells; skin; FETAL BOVINE SERUM; CELL; CULTURE; GROWTH; FIBRONECTIN; CONTRACTION; IMPROVE;
D O I
10.3390/ijms241612537
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Tissue-engineered skin substitutes (TESs) are used as a treatment for severe burn injuries. Their production requires culturing both keratinocytes and fibroblasts. The methods to grow these cells have evolved over the years, but bovine serum is still commonly used in the culture medium. Because of the drawbacks associated with the use of serum, it would be advantageous to use serum-free media for the production of TESs. In a previous study, we developed a serum-free medium (Surge SFM) for the culture of keratinocytes. Herein, we tested the use of this medium, together with a commercially available serum-free medium for fibroblasts (Prime XV), to produce serum-free TESs. Our results show that serum-free TESs are macroscopically and histologically similar to skin substitutes produced with conventional serum-containing media. TESs produced with either culture media expressed keratin 14, Ki-67, transglutaminase 1, filaggrin, type I and IV collagen, and fibronectin comparably. Mechanical properties, such as contraction and tensile strength, were comparable between TESs cultured with and without serum. Serum-free TESs were also successfully grafted onto athymic mice for a six-month period. In conclusion, Surge SFM and Prime XV serum-free media could be used to produce high quality clinical-grade skin substitutes.
引用
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页数:16
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