Decidual leukocytes respond to African lineage Zika virus infection with mild anti-inflammatory changes during acute infection in rhesus macaques

被引:0
作者
Koenig, Michelle R. [1 ]
Vazquez, Jessica [2 ]
Leyva Jaimes, Fernanda B. [2 ]
Mitzey, Ann M. [1 ]
Stanic, Aleksandar K. [2 ]
Golos, Thaddeus G. [1 ,2 ,3 ]
机构
[1] Univ Wisconsin Madison, Dept Comparat Biosci, Madison, WI 53706 USA
[2] Univ Wisconsin Madison, Dept Obstet & Gynecol, Madison, WI 53706 USA
[3] Univ Wisconsin Madison, Wisconsin Natl Primate Res Ctr, Madison, WI 53706 USA
基金
美国国家卫生研究院;
关键词
Zika virus; decidua; inflammation; immunome; rhesus macaque; pregnancy; NATURAL-KILLER-CELLS; MEMORY T-CELLS; NK CELLS; HLA-G; PREGNANCY OUTCOMES; HUMAN MONOCYTES; POPULATIONS; ACTIVATION; EXPRESSION; MACROPHAGES;
D O I
10.3389/fimmu.2024.1363169
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Zika virus (ZIKV) can be vertically transmitted during pregnancy resulting in a range of adverse pregnancy outcomes. The decidua is commonly found to be infected by ZIKV, yet the acute immune response to infection remains understudied in vivo. We hypothesized that in vivo African-lineage ZIKV infection induces a pro-inflammatory response in the decidua. To test this hypothesis, we evaluated the decidua in pregnant rhesus macaques within the first two weeks following infection with an African-lineage ZIKV and compared our findings to gestationally aged-matched controls. Decidual leukocytes were phenotypically evaluated using spectral flow cytometry, and cytokines and chemokines were measured in tissue homogenates from the decidua, placenta, and fetal membranes. The results of this study did not support our hypothesis. Although ZIKV RNA was detected in the decidual tissue samples from all ZIKV infected dams, phenotypic changes in decidual leukocytes and differences in cytokine profiles suggest that the decidua undergoes mild anti-inflammatory changes in response to that infection. Our findings emphasize the immunological state of the gravid uterus as a relatively immune privileged site that prioritizes tolerance of the fetus over mounting a pro-inflammatory response to clear infection.
引用
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页数:20
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