Production of D-tagatose, bioethanol, and microbial protein from the dairy industry by-product whey powder using an integrated bioprocess
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作者:
Ma, Wei
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Shandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Shandong, Peoples R ChinaShandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Shandong, Peoples R China
Ma, Wei
[1
]
Li, Fengyi
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Shandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Shandong, Peoples R ChinaShandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Shandong, Peoples R China
Li, Fengyi
[1
]
Li, Longyue
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Shandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Shandong, Peoples R ChinaShandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Shandong, Peoples R China
Li, Longyue
[1
]
Li, Bin
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Shandong Henglu Biotechnol Co Ltd, Jinan, Shandong, Peoples R ChinaShandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Shandong, Peoples R China
Li, Bin
[2
]
Niu, Kangle
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Shandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Shandong, Peoples R ChinaShandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Shandong, Peoples R China
Niu, Kangle
[1
]
Liu, Qinghua
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Shandong Henglu Biotechnol Co Ltd, Jinan, Shandong, Peoples R ChinaShandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Shandong, Peoples R China
Liu, Qinghua
[2
]
Han, Laichuang
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Jiangnan Univ, Sch Biotechnol, Wuxi, Jiangsu, Peoples R ChinaShandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Shandong, Peoples R China
Han, Laichuang
[3
]
Han, Lijuan
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Shandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Shandong, Peoples R ChinaShandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Shandong, Peoples R China
Han, Lijuan
[1
]
Fang, Xu
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Shandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Shandong, Peoples R China
Rongcheng Huihai Chuangda Biotechnol Co Ltd, Weihai, Shandong, Peoples R ChinaShandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Shandong, Peoples R China
Fang, Xu
[1
,4
]
机构:
[1] Shandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Shandong, Peoples R China
[2] Shandong Henglu Biotechnol Co Ltd, Jinan, Shandong, Peoples R China
[3] Jiangnan Univ, Sch Biotechnol, Wuxi, Jiangsu, Peoples R China
[4] Rongcheng Huihai Chuangda Biotechnol Co Ltd, Weihai, Shandong, Peoples R China
We designed and constructed a green and sustainable bioprocess to efficiently coproduce (D)-tagatose, bioethanol, and microbial protein from whey powder. First, a one-pot biosynthesis process involving lactose hydrolysis and (D)-galactose redox reactions for (D)-tagatose production was established in vitro via a three-enzyme cascade. Second, a nicotinamide adenine dinucleotide phosphate-dependent galactitol dehydrogenase mutant, D36A/I37R, based on the nicotinamide adenine dinucleotide-dependent polyol dehydrogenase from Paracoccus denitrificans was created through rational design and screening. Moreover, an NADPH recycling module was created in the oxidoreductive pathway, and the tagatose yield increased by 3.35-fold compared with that achieved through the pathway without the cofactor cycle. The reaction process was accelerated using an enzyme assembly with a glycine-serine linker, and the tagatose production rate was 9.28-fold higher than the initial yield. Finally, Saccharomyces cerevisiae was introduced into the reaction solution, and 266.5 g of (D)-tagatose, 162.6 g of bioethanol, and 215.4 g of dry yeast (including 38% protein) were obtained from 1 kg of whey powder (including 810 g lactose). This study provides a promising sustainable process for functional food ((D)-tagatose) production. Moreover, this process fully utilized whey powder, demonstrating good atom economy.
机构:
Joint BioEnergy Inst, Emeryville, CA 94608 USA
Sandia Natl Labs, Dept Biomat & Biomfg, Livermore, CA 94550 USAJoint BioEnergy Inst, Emeryville, CA 94608 USA
Garcia, Valentina E.
Pidatala, Venkataramana
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Joint BioEnergy Inst, Emeryville, CA 94608 USA
Lawrence Berkeley Natl Lab, Biol Syst & Engn Div, Berkeley, CA 94720 USAJoint BioEnergy Inst, Emeryville, CA 94608 USA
Pidatala, Venkataramana
Barcelos, Carolina A.
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Lawrence Berkeley Natl Lab, Biol Syst & Engn Div, Berkeley, CA 94720 USA
Adv Biofuels & Bioprod Proc Dev Unit, Emeryville, CA 94608 USAJoint BioEnergy Inst, Emeryville, CA 94608 USA
Barcelos, Carolina A.
Liu, Dupeng
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Lawrence Berkeley Natl Lab, Biol Syst & Engn Div, Berkeley, CA 94720 USA
Adv Biofuels & Bioprod Proc Dev Unit, Emeryville, CA 94608 USAJoint BioEnergy Inst, Emeryville, CA 94608 USA
Liu, Dupeng
Otoupal, Peter
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机构:
Joint BioEnergy Inst, Emeryville, CA 94608 USA
Sandia Natl Labs, Dept Biomat & Biomfg, Livermore, CA 94550 USAJoint BioEnergy Inst, Emeryville, CA 94608 USA
Otoupal, Peter
Wendt, Oliver
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Joint BioEnergy Inst, Emeryville, CA 94608 USAJoint BioEnergy Inst, Emeryville, CA 94608 USA
Wendt, Oliver
Choudhary, Hemant
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机构:
Joint BioEnergy Inst, Emeryville, CA 94608 USA
Sandia Natl Labs, Dept Bioresources & Environm Secur, Livermore, CA 94550 USAJoint BioEnergy Inst, Emeryville, CA 94608 USA
Choudhary, Hemant
Sun, Ning
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机构:
Lawrence Berkeley Natl Lab, Biol Syst & Engn Div, Berkeley, CA 94720 USA
Adv Biofuels & Bioprod Proc Dev Unit, Emeryville, CA 94608 USAJoint BioEnergy Inst, Emeryville, CA 94608 USA
Sun, Ning
Eudes, Aymerick
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Joint BioEnergy Inst, Emeryville, CA 94608 USA
Lawrence Berkeley Natl Lab, Environm Genom & Syst Biol Div, Berkeley, CA 94720 USAJoint BioEnergy Inst, Emeryville, CA 94608 USA
Eudes, Aymerick
Sundstrom, Eric R.
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Lawrence Berkeley Natl Lab, Biol Syst & Engn Div, Berkeley, CA 94720 USA
Adv Biofuels & Bioprod Proc Dev Unit, Emeryville, CA 94608 USAJoint BioEnergy Inst, Emeryville, CA 94608 USA
Sundstrom, Eric R.
Scheller, Henrik V.
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Joint BioEnergy Inst, Emeryville, CA 94608 USA
Lawrence Berkeley Natl Lab, Environm Genom & Syst Biol Div, Berkeley, CA 94720 USAJoint BioEnergy Inst, Emeryville, CA 94608 USA
Scheller, Henrik V.
Putnam, Daniel H.
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Joint BioEnergy Inst, Emeryville, CA 94608 USA
Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USAJoint BioEnergy Inst, Emeryville, CA 94608 USA
Putnam, Daniel H.
Mukhopadhyay, Aindrila
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Joint BioEnergy Inst, Emeryville, CA 94608 USA
Lawrence Berkeley Natl Lab, Biol Syst & Engn Div, Berkeley, CA 94720 USAJoint BioEnergy Inst, Emeryville, CA 94608 USA
Mukhopadhyay, Aindrila
Gladden, John M.
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Joint BioEnergy Inst, Emeryville, CA 94608 USA
Sandia Natl Labs, Dept Biomat & Biomfg, Livermore, CA 94550 USAJoint BioEnergy Inst, Emeryville, CA 94608 USA
Gladden, John M.
Simmons, Blake A.
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Joint BioEnergy Inst, Emeryville, CA 94608 USA
Lawrence Berkeley Natl Lab, Biol Syst & Engn Div, Berkeley, CA 94720 USAJoint BioEnergy Inst, Emeryville, CA 94608 USA
Simmons, Blake A.
Rodriguez, Alberto
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Joint BioEnergy Inst, Emeryville, CA 94608 USA
Sandia Natl Labs, Dept Biomat & Biomfg, Livermore, CA 94550 USAJoint BioEnergy Inst, Emeryville, CA 94608 USA
机构:
Korea Inst Sci & Technol KIST, Clean Energy Res Ctr, Seoul 02792, South Korea
Korea Univ, Dept Biotechnol, Seoul 02841, South KoreaKorea Inst Sci & Technol KIST, Clean Energy Res Ctr, Seoul 02792, South Korea
Lee, Yeon Ji
Moon, Byeong Cheul
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Korea Inst Sci & Technol KIST, Water Cycle Res Ctr, Seoul 02792, South KoreaKorea Inst Sci & Technol KIST, Clean Energy Res Ctr, Seoul 02792, South Korea
Moon, Byeong Cheul
Lee, Dong Ki
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Korea Inst Sci & Technol KIST, Clean Energy Res Ctr, Seoul 02792, South Korea
Univ Sci & Technol, KIST Sch, Div Energy & Environm Technol, Seoul 02792, South KoreaKorea Inst Sci & Technol KIST, Clean Energy Res Ctr, Seoul 02792, South Korea
Lee, Dong Ki
Ahn, Jung Ho
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Korea Inst Sci & Technol KIST, Clean Energy Res Ctr, Seoul 02792, South Korea
Univ Sci & Technol, KIST Sch, Div Energy & Environm Technol, Seoul 02792, South KoreaKorea Inst Sci & Technol KIST, Clean Energy Res Ctr, Seoul 02792, South Korea
Ahn, Jung Ho
Gong, Gyeongtaek
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Korea Inst Sci & Technol KIST, Clean Energy Res Ctr, Seoul 02792, South Korea
Univ Sci & Technol, KIST Sch, Div Energy & Environm Technol, Seoul 02792, South KoreaKorea Inst Sci & Technol KIST, Clean Energy Res Ctr, Seoul 02792, South Korea
Gong, Gyeongtaek
Um, Youngsoon
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机构:
Korea Inst Sci & Technol KIST, Clean Energy Res Ctr, Seoul 02792, South Korea
Univ Sci & Technol, KIST Sch, Div Energy & Environm Technol, Seoul 02792, South KoreaKorea Inst Sci & Technol KIST, Clean Energy Res Ctr, Seoul 02792, South Korea
Um, Youngsoon
Lee, Sun-Mi
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机构:
Korea Univ, Dept Environm Sci & Ecol Engn, Seoul 02841, South KoreaKorea Inst Sci & Technol KIST, Clean Energy Res Ctr, Seoul 02792, South Korea
Lee, Sun-Mi
Kim, Kyoung Heon
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机构:
Korea Univ, Dept Biotechnol, Seoul 02841, South KoreaKorea Inst Sci & Technol KIST, Clean Energy Res Ctr, Seoul 02792, South Korea
Kim, Kyoung Heon
Ko, Ja Kyong
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机构:
Korea Inst Sci & Technol KIST, Clean Energy Res Ctr, Seoul 02792, South Korea
Univ Sci & Technol, KIST Sch, Div Energy & Environm Technol, Seoul 02792, South KoreaKorea Inst Sci & Technol KIST, Clean Energy Res Ctr, Seoul 02792, South Korea