Multiplexed CRISPR-based methods for pathogen nucleic acid detection

被引:4
作者
Lamb, Caitlin H. [1 ]
Kang, Brian [1 ]
Myhrvold, Cameron [1 ]
机构
[1] Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA
基金
美国国家卫生研究院;
关键词
CRISPR Pathogen detection Multiplexing Point-of-care Nucleic acid; amplification; CRISPR; Pathogen detection; Multiplexing Point-of-care; Nucleic acid amplification; DIAGNOSIS;
D O I
10.1016/j.cobme.2023.100471
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Bacterial and viral pathogens are devastating to human health and well-being. In many regions, dozens of pathogen species and variants co-circulate. Thus, it is important to detect many different species and variants of pathogens in a given sample through multiplexed detection methods. CRISPR-based nucleic acid detection has shown to be a promising step towards an easy-to-use, sensitive, specific, and high-throughput method to detect nucleic acids from DNA and RNA viruses and bacteria. Here, we review the current state of multiplexed nucleic acid detection methods with a focus on CRISPR-based methods. We also look toward the future of multiplexed pointof-care diagnostics.
引用
收藏
页数:10
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