Synthetic Pph6his Gene Confers Resistance to Cadmium Stress in Transgenic Tobacco

被引：0
作者：
Vershinina, Z. R. [1 ,2 ]
Maslennikova, D. R. [1 ,2 ]
Chubukova, O. V. [1 ,2 ]
Khakimova, L. R. [1 ,2 ]
Mikhaylova, E. V. [1 ,2 ]
机构：
[1] Russian Acad Sci, Inst Biochem & Genet, Ufa Fed Res Ctr, Ufa, Russia
[2] Ufa State Petr Technocol Univ, Ufa, Russia
来源：
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY | 2023年 / 70卷 / 04期
关键词：
Nicotiana tabacum; glutathione; ascorbate; phytochelatin; photosynthetic pigments; heavy metals; phytoremediation; cadmium; PHOTOSYNTHETIC PIGMENTS; PHYTOCHELATIN SYNTHASE; PLANT-RESPONSE; GLUTATHIONE; GROWTH; ACCUMULATION; TOLERANCE; ACID;
D O I：
10.1134/S1021443723600216
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Phytochelatins play an important role in plant resistance to cadmium. The pph6his gene encoding a peptide analog of natural phytochelatin with an & alpha;-peptide bond MetHys(6)[& alpha;-Glu(Cis)](6)Gly was synthesized and used for genetic transformation of tobacco (Nicotiana tabacum L., variety Petit Havana, line SR1). The effect of cadmium on the accumulation of biomass and the content of glutathione, ascorbate, pigments (chlorophyll a, b and carotenoids) and malondialdehyde (MDA) in transgenic plants was evaluated. It was shown that plants of line 76 are able to grow and develop stably in the presence of up to 100 & mu;mol of cadmium. These plants have also accumulated enough cadmium in the aerial parts to be used in phytoremediation. The created genetic construct can be used for transformation of various plant species.
引用
收藏
页数：11
相关论文
共 50 条
[41]   Expression of rice chitinase gene in transgenic eggplant confers resistance to fungal wilts [J].
Singh, Deepali ;
Haicour, Robert ;
Sihachakr, Darasinh ;
Rajam, Manchikatla Venkat .
INDIAN JOURNAL OF BIOTECHNOLOGY, 2015, 14 (02) :233-240
[42]   TaCIPK29, a CBL-Interacting Protein Kinase Gene from Wheat, Confers Salt Stress Tolerance in Transgenic Tobacco [J].
Deng, Xiaomin ;
Hu, Wei ;
Wei, Shuya ;
Zhou, Shiyi ;
Zhang, Fan ;
Han, Jiapeng ;
Chen, Lihong ;
Li, Yin ;
Feng, Jialu ;
Fang, Bin ;
Luo, Qingchen ;
Li, Shasha ;
Liu, Yunyi ;
Yang, Guangxiao ;
He, Guangyuan .
PLOS ONE, 2013, 8 (07)
[43]   Transgenic Tobacco with the BADH Gene Shows Enhanced Photosynthesis Resistance to Drought Stress Induced by PEG-6000 [J].
Wang, Guiping ;
Yang, Xinghong ;
Xue, Xiaomin .
AGRONOMY-BASEL, 2024, 14 (04)
[44]   A type 2 metallothionein (SbMT-2) gene cloned from Salicornia brachiata confers enhanced Zn stress-tolerance in transgenic tobacco by transporting Zn2+and maintaining photosynthesis efficacy [J].
Patel, Manish Kumar ;
Pandey, Sonika ;
Patel, Jaykumar ;
Mishra, Avinash .
ENVIRONMENTAL AND EXPERIMENTAL BOTANY, 2021, 191
[45]   Enhanced stress-tolerance of transgenic tobacco plants expressing a human dehydroascorbate reductase gene [J].
Kwon, SY ;
Choi, SM ;
Ahn, YO ;
Lee, HS ;
Lee, HB ;
Park, YM ;
Kwak, SS .
JOURNAL OF PLANT PHYSIOLOGY, 2003, 160 (04) :347-353
[46]   Pearl Millet Aquaporin Gene PgPIP2;6 Improves Abiotic Stress Tolerance in Transgenic Tobacco [J].
Reddy, Palakolanu Sudhakar ;
Dhaware, Mahamaya G. ;
Sivasakthi, Kaliamoorthy ;
Divya, Kummari ;
Nagaraju, Marka ;
Cindhuri, Katamreddy Sri ;
Kishor, Polavarapu Bilhan Kavi ;
Bhatnagar-Mathur, Pooja ;
Vadez, Vincent ;
Sharma, Kiran K. .
FRONTIERS IN PLANT SCIENCE, 2022, 13
[47]   Gentian lipid transfer protein homolog with antimicrobial properties confers resistance to Botrytis cinerea in transgenic tobacco [J].
Kiba, Akinori ;
Nakatsuka, Takashi ;
Yamamura, Saburo ;
Nishihara, Masahiro .
PLANT BIOTECHNOLOGY, 2012, 29 (01) :95-101
[48]   Root growth in transgenic tobacco plants with overexpression of the PtrXTH1 gene encoding xyloglucan endotransglycosylase under abiotic stress [J].
Berezhneva, Zoya A. ;
Musin, Khalit G. ;
Kuluev, Bulat R. .
IZVESTIYA VUZOV-PRIKLADNAYA KHIMIYA I BIOTEKHNOLOGIYA, 2024, 14 (04)
[49]   A Versatile Peroxidase from the Fungus Bjerkandera adusta Confers Abiotic Stress Tolerance in Transgenic Tobacco Plants [J].
Sofia Hernandez-Bueno, Nancy ;
Suarez-Rodriguez, Ramon ;
Balcazar-Lopez, Edgar ;
Luis Folch-Mallol, Jorge ;
Augusto Ramirez-Trujillo, Jose ;
Iturriaga, Gabriel .
PLANTS-BASEL, 2021, 10 (05)
[50]   CfAPX, a cytosolic ascorbate peroxidase gene from Cryptomeria fortunei, confers tolerance to abiotic stress in transgenic Arabidopsis [J].
Zhang, Yingting ;
Yang, Liwei ;
Zhang, Meng ;
Yang, Junjie ;
Cui, Jiebing ;
Hu, Hailiang ;
Xu, Jin .
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2022, 172 :167-179
12345