Synthetic Pph6his Gene Confers Resistance to Cadmium Stress in Transgenic Tobacco

被引：0
|
作者：
Vershinina, Z. R. [1 ,2 ]
Maslennikova, D. R. [1 ,2 ]
Chubukova, O. V. [1 ,2 ]
Khakimova, L. R. [1 ,2 ]
Mikhaylova, E. V. [1 ,2 ]
机构：
[1] Russian Acad Sci, Inst Biochem & Genet, Ufa Fed Res Ctr, Ufa, Russia
[2] Ufa State Petr Technocol Univ, Ufa, Russia
来源：
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY | 2023年 / 70卷 / 04期
关键词：
Nicotiana tabacum; glutathione; ascorbate; phytochelatin; photosynthetic pigments; heavy metals; phytoremediation; cadmium; PHOTOSYNTHETIC PIGMENTS; PHYTOCHELATIN SYNTHASE; PLANT-RESPONSE; GLUTATHIONE; GROWTH; ACCUMULATION; TOLERANCE; ACID;
D O I：
10.1134/S1021443723600216
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Phytochelatins play an important role in plant resistance to cadmium. The pph6his gene encoding a peptide analog of natural phytochelatin with an & alpha;-peptide bond MetHys(6)[& alpha;-Glu(Cis)](6)Gly was synthesized and used for genetic transformation of tobacco (Nicotiana tabacum L., variety Petit Havana, line SR1). The effect of cadmium on the accumulation of biomass and the content of glutathione, ascorbate, pigments (chlorophyll a, b and carotenoids) and malondialdehyde (MDA) in transgenic plants was evaluated. It was shown that plants of line 76 are able to grow and develop stably in the presence of up to 100 & mu;mol of cadmium. These plants have also accumulated enough cadmium in the aerial parts to be used in phytoremediation. The created genetic construct can be used for transformation of various plant species.
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页数：11
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