Integrative mRNA-miRNA interaction analysis associated with the immune response of Strongylocentrotus intermedius to Vibrio harveyi infection

被引：10

作者：

Hao, Pengfei ^{[1
]}

Han, Lingshu ^{[1
,2
]}

Quan, Zijiao ^{[1
]}

Jin, Xin ^{[1
]}

Li, Yuanxin ^{[1
]}

Wu, Yanglei ^{[1
]}

Zhang, Xianglei ^{[1
]}

Wang, Wenpei ^{[1
]}

Gao, Chuang ^{[1
]}

Wang, Luo ^{[1
]}

Wang, Heng ^{[1
]}

Zhang, Weijie ^{[1
]}

Chang, Yaqing ^{[1
]}

Ding, Jun ^{[1
,3
]}

机构：

[1] Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chin, Minist Agr & Rural Affairs, Dalian 116023, Liaoning, Peoples R China

[2] Ningbo Univ, Ningbo 315832, Zhejiang, Peoples R China

[3] Dalian Ocean Univ, 52 Heishijiao Rd, Dalian 116023, Liaoning, Peoples R China

来源：

FISH & SHELLFISH IMMUNOLOGY | 2023年 / 134卷

关键词：

Strongylocentrotus intermedius; Vibrio harveyi; Transcriptome; mRNA-miRNA interaction; KEGG analysis; Temporal expression; TRANSCRIPTOME ANALYSIS; EXPRESSION; IDENTIFICATION; MICRORNAS; EVOLUTION; SUBUNIT;

D O I：

10.1016/j.fsi.2023.108577

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Strongylocentrotus intermedius is one of the most economically valuable sea urchin species in China and has experienced mass mortality owing to outbreaks of bacterial diseases such as black mouth disease. This has caused serious economic losses to the sea urchin farming industry. To investigate the immune response mechanism of S. intermedius with different tube feet colors in response to Vibrio harveyi infection, we examined the different tube feet-colored S. intermedius under V. harveyi challenge and compared their transcriptome and microRNA (miRNA) profiles using RNA-Seq. We obtained 1813 differentially expressed genes (DEGs), 28 DE miRNAs, and 303 DE miRNA-DEG pairs in different tube feet-colored S. intermedius under V. harveyi challenge. Kyoto Ency-clopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that the most significant DEGs were associated with the Notch signaling and phagosome pathways. The target genes of immune-related miRNAs (miR-71, miR-184, miR-193) and genes (CALM1, SPSB4, DMBT, CSRP1) in S. intermedius were predicted and validated. This study provides insight into the molecular mechanisms that regulate genes involved in the immune response of S. intermedius infected with V. harveyi.

引用

页数：16

共 85 条

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