Paralog editing tunes rice stomatal density to maintain photosynthesis and improve drought tolerance

被引:24
作者
Karavolias, Nicholas G. [1 ,2 ]
Patel-Tupper, Dhruv [1 ,3 ]
Seong, Kyungyong [1 ]
Tjahjadi, Michelle [2 ]
Gueorguieva, Gloria-Alexandra [1 ,2 ]
Tanaka, Jaclyn [2 ]
Cruz, Ana Gallegos [2 ]
Lieberman, Samantha [2 ]
Litvak, Lillian [2 ]
Dahlbeck, Douglas [1 ,2 ]
Cho, Myeong-Je [2 ]
Niyogi, Krishna K. [1 ,3 ]
Staskawicz, Brian J. [1 ,2 ]
机构
[1] Univ Calif Berkeley, Plant & Microbial Biol Dept, Berkeley, CA 94720 USA
[2] Innovat Genom Inst, Berkeley, CA 94704 USA
[3] Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA
基金
美国国家卫生研究院; 美国国家科学基金会;
关键词
MULTIPLE SEQUENCE ALIGNMENT; GENETIC MANIPULATION; SECRETORY PEPTIDE; HIGH-TEMPERATURE; CONDUCTANCE; YIELD; TRAITS; STRESS; EPFL9;
D O I
10.1093/plphys/kiad183
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Rice (Oryza sativa) is of paramount importance for global nutrition, supplying at least 20% of global calories. However, water scarcity and increased drought severity are anticipated to reduce rice yields globally. We explored stomatal developmental genetics as a mechanism for improving drought resilience in rice while maintaining yield under climate stress. CRISPR/Cas9-mediated knockouts of the positive regulator of stomatal development STOMAGEN and its paralog EPIDERMAL PATTERNING FACTOR-LIKE10 (EPFL10) yielded lines with similar to 25% and 80% of wild-type stomatal density, respectively. epfl10 lines with moderate reductions in stomatal density were able to conserve water to similar extents as stomagen lines but did not suffer from the concomitant reductions in stomatal conductance, carbon assimilation, or thermoregulation observed in stomagen knockouts. Moderate reductions in stomatal density achieved by editing EPFL10 present a climate-adaptive approach for safeguarding yield in rice. Editing the paralog of STOMAGEN in other species may provide a means for tuning stomatal density in agriculturally important crops beyond rice.
引用
收藏
页码:1168 / 1182
页数:15
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