Convalescent human IgG, but not IgM, from COVID-19 survivors confers dose-dependent protection against SARS-CoV-2 replication and disease in hamsters

被引：0

作者：

King, Hannah A. D. ^{[1
,2
,3
]}

Dussupt, Vincent ^{[1
,2
,3
]}

Mendez-Rivera, Letzibeth ^{[1
,2
,3
]}

Slike, Bonnie M. M. ^{[1
,2
,3
]}

Tran, Ursula ^{[1
,2
,3
]}

Jackson, Nathan D. D. ^{[1
,2
,3
]}

Barkei, Erica ^{[4
]}

Zemil, Michelle ^{[1
,2
,3
]}

Tourtellott-Fogt, Emily ^{[1
,2
,3
]}

Kuklis, Caitlin H. H. ^{[5
]}

Soman, Sandrine ^{[5
]}

Ahmed, Aslaa ^{[5
]}

Porto, Maciel ^{[6
]}

Kitajewski, Christopher ^{[6
]}

Spence, Brittany ^{[6
]}

Benetiene, Dalia ^{[6
]}

Wieczorek, Lindsay ^{[1
,2
,3
]}

Kar, Swagata ^{[6
]}

Gromowski, Gregory ^{[5
]}

Polonis, Victoria R. R. ^{[1
]}

Krebs, Shelly J. J. ^{[1
,2
,3
]}

Modjarrad, Kayvon ^{[2
]}

Bolton, Diane L. L. ^{[1
,2
,3
]}

机构：

[1] Walter Reed Army Inst Res, US Mil HIV Res Program, Silver Spring, MD 20910 USA

[2] WRAIR, Emerging Infect Dis Branch, Silver Spring, MD 20910 USA

[3] Henry M Jackson Fdn Advancement Mil Med, Bethesda, MD 20817 USA

[4] WRAIR, Vet Pathol Branch, Silver Spring, MD USA

[5] WRAIR, Viral Dis Branch, Silver Spring, MD USA

[6] BIOQUAL Inc, Rockville, MD USA

来源：

FRONTIERS IN IMMUNOLOGY | 2023年 / 14卷

关键词：

SARS-CoV-2; antibody; passive transfer; IgM; IgG; hamster; ANTIBODIES; RESPONSES;

D O I：

10.3389/fimmu.2023.1138629

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

IntroductionAntibody therapeutic strategies have served an important role during the COVID-19 pandemic, even as their effectiveness has waned with the emergence of escape variants. Here we sought to determine the concentration of convalescent immunoglobulin required to protect against disease from SARS-CoV-2 in a Syrian golden hamster model. MethodsTotal IgG and IgM were isolated from plasma of SARS-CoV-2 convalescent donors. Dose titrations of IgG and IgM were infused into hamsters 1 day prior to challenge with SARS-CoV-2 Wuhan-1. ResultsThe IgM preparation was found to have similar to 25-fold greater neutralization potency than IgG. IgG infusion protected hamsters from disease in a dose-dependent manner, with detectable serum neutralizing titers correlating with protection. Despite a higher in vitro neutralizing potency, IgM failed to protect against disease when transferred into hamsters. DiscussionThis study adds to the growing body of literature that demonstrates neutralizing IgG antibodies are important for protection from SARS-CoV-2 disease, and confirms that polyclonal IgG in sera can be an effective preventative strategy if the neutralizing titers are sufficiently high. In the context of new variants, against which existing vaccines or monoclonal antibodies have reduced efficacy, sera from individuals who have recovered from infection with the emerging variant may potentially remain an efficacious tool.

引用

页数：14