Comparative analysis of the volatile constituents of Anvillea garcinii subsp. radiata obtained by Head-Space Solid-Phase Micro Extraction (HS-SPME) and classic hydrodistillation and antioxidant activity of essential oil

This research aims to extract the essential oil (EO) and a volatile fraction (VF) from Anvillea garcinii subsp. radiata obtained by hydrodistillation (HD) and Head-Space Solid-Phase Micro Extraction (HS-SPME) methods, respectively as well as to examine the antioxidant activity of isolated EO. Gas chromatography (GC) and GC-mass spectroscopy (GC-MS) allowed the identification of 35 and 26 volatile components, representing respectively 90.7% and 91.6% of the total compositions of EO and VF. Quantitative and qualitative differences in two chemoprofiles were observed. Cis 8-acetoxychrysantenyl acetate (30.8%), cis-chrysanthenyl acetate (15.5%) and 6-oxocyclonerolidol (14.9%) were the major constituents of the EO, whereas 6-oxocyclonerolidol (53.9%) and 6-hydroxycyclonerolidol (18.1%) were the main components of HS-SPME extract. The antioxidative activity of the isolated EO was determined by using two different methods: 2,2-diphenyl-1-picrylhydrazyl (DPPH center dot) radical activity assay and the beta-carotene bleaching (BCB) test. The results showed that EO expresses satisfactory antioxidant activity with IC50 values of 270.53 and 181.92 mu g/ml using DPPH and BCB assays, respectively. To our knowledge, this is the first report describing the VF obtained by HS-SPME-GC-MS method and the antioxidant activity of this species.