Endothelial Progenitor-Cell-Derived Exosomes Induced by Astragaloside IV Accelerate Type I Diabetic-wound Healing via the PI3K/AKT/mTOR Pathway in Rats

被引：9

作者：

Xiong, Wu ^{[1
]}

Bai, Xue ^{[2
]}

Zhang, Xi ^{[3
,4
]}

Lei, Huajuan ^{[5
]}

Xiao, Hui ^{[2
]}

Zhang, Luyao ^{[6
]}

Xiao, Yuting ^{[6
]}

Yang, Qianpei ^{[6
]}

Zou, Xiaoling ^{[2
]}

机构：

[1] Hunan Univ Chinese Med, Affiliated Hosp 1, Dept Burns & Plast Surg, Changsha 410007, Hunan, Peoples R China

[2] Hunan Univ Chinese Med, Affiliated Hosp 1, Dept Endocrinol, Changsha 410007, Hunan, Peoples R China

[3] Hunan Prov Brain Hosp, Dept Sci Res, Changsha 410007, Hunan, Peoples R China

[4] Hunan Univ Chinese Med, Clin Med Sch, Changsha 410007, Hunan, Peoples R China

[5] Hunan Univ Chinese Med, Affiliated Hosp 1, Dept Anesthesiol, Changsha 410007, Hunan, Peoples R China

[6] Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Changsha 410208, Hunan, Peoples R China

来源：

FRONTIERS IN BIOSCIENCE-LANDMARK | 2023年 / 28卷 / 11期

基金：

湖南省自然科学基金; 中国国家自然科学基金;

关键词：

astragaloside IV; PI3K/AKT/mTOR; endothelial progenitor cells; exosome; angiogenesis; OXIDATIVE STRESS; FOOT ULCER; ANGIOGENESIS; INFLAMMATION; MELLITUS;

D O I：

10.31083/j.fbl2811282

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Objective: We explore the effects of endothelial progenitor cell (EPC)-derived exosomes (EPCexos) and of astragaloside IV (ASIV)stimulated EPCexos (ASIV-EPCexos) on type I diabetic-wound healing, and determine the basic molecular mechanisms of action. Methods: EPCs were exposed to different concentrations of ASIV to generate ASIV-EPCexos. A chronic-wound healing model involving streptozotocin-stimulated diabetic rats was established. These rats were treated with EPCexos, ASIV-EPCexos, rapamycin, and wortmannin. Wound healing was evaluated by direct photographic observation, hematoxylin and eosin staining, and Masson's trichrome staining. Results: ASIV treatment increased the abilities of EPCs (e.g., proliferation), as well as exosome secretion. EPCexo showed a "cup holder" like structure. Treatment with ASIV-EPCexos increased the wound-healing rate, collagen-deposition area, bromodeoxyuridine uptake, VEGF expression, and the number of CD31- and alpha SMA- positive cells, whereas decreased epidermal thickness and CD45 expression. The expression of the PI3K/AKT/mTOR pathway increased, whereas the expression of inflammatory factor decreased. However, rapamycin and wortmannin reversed these changes. Conclusions: ASIV-EPCexos may accelerate type I diabetic-wound healing via the PI3K/AKT/mTOR pathway. This study may lay the foundation for new clinical treatment options for patients with type I diabetic wounds.

引用

页数：14

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