Combining Metabolic Engineering and Lipid Droplet Storage Engineering for Improved α-Bisabolene Production in Yarrowia Lipolytica

被引：15

作者：

Lu, Zhihui ^{[1
]}

Wang, Yaping ^{[1
]}

Li, Zhuo ^{[1
]}

Zhang, Yahui ^{[1
]}

He, Sicheng ^{[1
]}

Zhang, Ziyuan ^{[1
]}

Leong, Susanna ^{[2
]}

Wong, Adison ^{[2
]}

Zhang, Cui-ying ^{[1
]}

Yu, Aiqun ^{[1
]}

机构：

[1] Tianjin Univ Sci & Technol, Coll Biotechnol, State Key Lab Food Nutr & Safety, Key Lab Ind Fermentat Microbiol,Minist Educ,Tianji, Tianjin 300457, Peoples R China

[2] Singapore Inst Technol, Food Chem & Biotechnol Cluster, Singapore 138683, Singapore

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2023年 / 71卷 / 30期

关键词：

Y; lipolytica; sesquiterpenoid; bisabolene; mevalonate pathway; metabolic engineering; lipid droplet; SACCHAROMYCES-CEREVISIAE; BIOSYNTHESIS; EXPRESSION;

D O I：

10.1021/acs.jafc.3c02472

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Bisaboleneis a bioactive sesquiterpene with a wide range of applicationsin food, cosmetics, medicine, and aviation fuels. Microbial productionoffers a green, efficient, and sustainable alternative. In this study,we focused on improving the titers of & alpha;-bisabolene in Yarrowia lipolytica by applying two strategies, (i) optimizingthe metabolic flux of & alpha;-bisabolene biosynthetic pathway and(ii) sequestering & alpha;-bisabolene in lipid droplet, thus alleviatingits inherent toxicity to host cells. We showed that overexpressionof DGA1 and OLE1 to increase lipidcontent and unsaturated fatty acid levels was essential for boostingthe & alpha;-bisabolene synthesis when supplemented with auxiliarycarbon sources. The final engineered strain Po1g & alpha;B10 produced1954.3 mg/L & alpha;-bisabolene from the waste cooking oil under shakeflask fermentation, which was 96-fold higher than the control strainPo1g & alpha;B0. At the time of writing, our study represents the highestreported & alpha;-bisabolene titer in the engineered Y. lipolytica cell factory. This work describes novel strategies to improve thebioproduction of & alpha;-bisabolene that potentially may be applicablefor other high-value terpene products.

引用

页码：11534 / 11543

页数：10

共 51 条

[1] The yeast lipin orthologue Pah1p is important for biogenesis of lipid droplets [J].