Mycobacterium tuberculosis Rv1916 is an Acetyl-CoA-Binding Protein

被引：1
作者：
Huang, Evelyn Yu-Wen [1 ,2 ]
Kwai, Brooke X. C. [1 ,2 ,3 ,4 ]
Bhusal, Ram Prasad [3 ,5 ,6 ]
Bashiri, Ghader [7 ]
Leung, Ivanhoe K. H. [1 ,2 ,3 ]
机构：
[1] Univ Melbourne, Sch Chem, 30 Flemington Rd, Parkville, Vic 3052, Australia
[2] Univ Melbourne, Mol Sci & Biotechnol Inst Bio21, 30 Flemington Rd, Parkville, Vic 3052, Australia
[3] Univ Auckland, Sch Chem Sci, Private Bag 92019, Auckland 1142, New Zealand
[4] Monash Univ, Monash Inst Pharmaceut Sci, 381 Royal Parade, Parkville, Vic 3052, Australia
[5] Monash Biomed Discovery Inst, Clayton, Vic 3800, Australia
[6] Monash Univ, Dept Biochem & Mol Biol, Clayton, Vic 3800, Australia
[7] Univ Auckland, Sch Biol Sci, Private Bag 92019, Auckland 1142, New Zealand
来源：
CHEMBIOCHEM | 2023年 / 24卷 / 14期
关键词：
isocitrate lyase; glyoxylate shunt; Rv1916; acyl-CoA-binding protein; Mycobacterium tuberculosis; ISOCITRATE LYASE; RECOMBINANT PROTEINS; SUCCINYLATION; EXPRESSION; RESISTANCE; CATALYSIS; NMR;
D O I：
10.1002/cbic.202300162
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Isocitrate lyase (ICL) isoform 2 is an essential enzyme for some clinical Mycobacterium tuberculosis (Mtb) strains during infection. In the laboratory Mtb strain H37Rv, the icl2 gene encodes two distinct gene products - Rv1915 and Rv1916 - due to a frameshift mutation. This study aims to characterise these two gene products to understand their structure and function. While we were unable to produce Rv1915 recombinantly, soluble Rv1916 was obtained with sufficient yield for characterisation. Kinetic studies using UV-visible spectrophotometry and H-1-NMR spectroscopy showed that recombinant Rv1916 does not possess isocitrate lyase activity, while waterLOGSY binding experiments demonstrated that it could bind acetyl-CoA. Finally, X-ray crystallography revealed structural similarities between Rv1916 and the C-terminal domain of ICL2. Considering the probable differences between full-length ICL2 and the gene products Rv1915 and Rv1916, care must be taken when using Mtb H37Rv as a model organism to study central carbon metabolism.
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页数：7
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