Three-Dimensional Spheroid Culture of Human Mesenchymal Stem Cells: Offering Therapeutic Advantages and In Vitro Glimpses of the In Vivo State

被引:48
作者
Yen, B. Linju [1 ,5 ]
Hsieh, Chen-Chan [1 ,2 ]
Hsu, Pei-Ju [1 ]
Chang, Chia-Chi [1 ,3 ]
Wang, Li-Tzu [4 ]
Yen, Men-Luh [4 ,6 ]
机构
[1] Natl Hlth Res Inst NHRI, Regenerat Med Res Grp, Inst Cellular & Syst Med, Zhunan, Taiwan
[2] Natl Tsing Hua Univ, Inst Mol Med, Hsinchu, Taiwan
[3] Natl Def Med Ctr NDMC, Grad Inst Life Sci, Taipei, Taiwan
[4] NTU, Natl Taiwan Univ NTU Hosp & Coll Med, Dept Obstet & Gynecol, Taipei, Taiwan
[5] NHRI, Inst Cellular & Syst Med, 35 Keyan Rd, Zhunan 35053, Taiwan
[6] NTU, Dept Obstet Gynecol, NTUH & Coll Med, 1,Sect 1,Jen Ai Rd, Taipei 10051, Taiwan
关键词
3-dimensional cell culture; multicellular spheroid; human mesenchymal stem cells; multilineage differentiation; chondrogenesis; osteogenesis; paracrine factors; immunomodulation; angiogenesis; wound-healing; EXTRACELLULAR-MATRIX; STROMAL CELLS; OSTEOGENIC DIFFERENTIATION; CANCER CELLS; EXPRESSION; TRANSPLANTATION; EXPANSION; SURVIVAL; MSCS; IDENTIFICATION;
D O I
10.1093/stcltm/szad011
中图分类号
Q813 [细胞工程];
学科分类号
摘要
As invaluable as the standard 2-dimensional (2D) monolayer in vitro cell culture system has been, there is increasing evidence that 3-dimensional (3D) non-adherent conditions are more relevant to the in vivo condition. While one of the criteria for human mesenchymal stem cells (MSCs) has been in vitro plastic adherence, such 2D culture conditions are not representative of in vivo cell-cell and cell-extracellular matrix (ECM) interactions, which may be especially important for this progenitor/stem cell of skeletal and connective tissues. The 3D spheroid, a multicellular aggregate formed under non-adherent 3D in vitro conditions, may be particularly suited as an in vitro method to better understand MSC physiological processes, since expression of ECM and other adhesion proteins are upregulated in such a cell culture system. First used in embryonic stem cell in vitro culture to recapitulate in vivo developmental processes, 3D spheroid culture has grown in popularity as an in vitro method to mimic the 3-dimensionality of the native niche for MSCs within tissues/organs. In this review, we discuss the relevance of the 3D spheroid culture for understanding MSC biology, summarize the biological outcomes reported in the literature based on such this culture condition, as well as contemplate limitations and future considerations in this rapidly evolving and exciting area.
引用
收藏
页码:235 / 244
页数:10
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