The Essential Oil from Oliveria decumbens Vent. (Apiaceae) as Inhibitor of Breast Cancer Cell (MCF-7) Growth

被引:3
作者
Shariatzadeh, Mandana [1 ]
Karami, Akbar [2 ]
Moghadam, Ali [1 ]
Lotfi, Mahbobeh [1 ]
Maggi, Filippo [3 ]
Ebrahimie, Esmaeil [4 ,5 ,6 ]
机构
[1] Shiraz Univ, Inst Biotechnol, Shiraz 71441, Iran
[2] Shiraz Univ, Sch Agr, Dept Hort Sci, Shiraz 71441, Iran
[3] Univ Camerino, Chem Interdisciplinary Project ChIP Res Ctr, Sch Pharm, I-62032 Camerino, Italy
[4] La Trobe Univ, Sch Agr Biomed & Environm, Genom Res Platform, Melbourne, Vic 3000, Australia
[5] Univ Adelaide, Sch Anim & Vet Sci, Adelaide, SA 5371, Australia
[6] Univ Melbourne, Sch Biosci, Melbourne, Vic 3010, Australia
关键词
breast cancer; PTEN protein; Aurora kinase A; essential oil; apoptosis; Oliveria decumbens; CHEMICAL-COMPOSITION; ANTIOXIDANT ACTIVITY; TUMOR-SUPPRESSOR; PTEN; EXPRESSION; APOPTOSIS; L;
D O I
10.3390/ph16010059
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Oliveria decumbens Vent. is an aromatic and medicinal plant traditionally used in Iran for the treatment of infections, gastrointestinal diseases, cancer, and inflammation. This research was aimed at investigating the pharmacological potential of O. decumbens essential oil (OEO) and its main compounds, focusing on OEO's cytotoxic effects on MCF-7 breast cancer cells. OEO was obtained by hydro-distillation, and the chemical constituents were identified using GC-MS. Thymol, carvacrol, gamma-terpinene, and p-cymene were the main OEO constituents. When MCF-7 cells were treated with OEO, the expressions of genes related to apoptosis (BIM and Bcl-2), tumor suppression (PTEN), and cell growth inhibition (AURKA), were evaluated using real-time PCR. Moreover, molecular docking was used for studying in silico the interaction of OEO principal compounds with PTEN and AURKA. The expression of AURKA was significantly reduced since the OEO treatment enhanced the expression of PTEN. Through in silico molecular docking, it was revealed that thymol, carvacrol, p-cymene, and gamma-terpinene can activate PTEN and thus inhibit AURKA. Additionally, the DNA fragmentation assay, acridine orange/ethidium bromide (AO/EB) double-staining assay, and real-time PCR highlighted the fact that the OEO treatment could activate apoptosis and inhibit cell proliferation. Therefore, OEO is a viable candidate to be employed in the pharmaceutical industry, specifically as a possible agent for cancer therapy.
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页数:14
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