Multi-epitope vaccine design for hepatitis E virus based on protein ORF2 and ORF3

被引：1
|
作者：
Lu, Qiong [1 ,2 ]
Wu, Hao [1 ,2 ,3 ]
Meng, Jing [4 ]
Wang, Jiangyuan [5 ]
Wu, Jiajing [6 ]
Liu, Shuo [7 ,8 ]
Tong, Jincheng [1 ,2 ]
Nie, Jianhui [1 ,2 ]
Huang, Weijin [1 ,2 ]
机构：
[1] Natl Inst Food & Drug Control, Inst Biol Prod Control, Div HIV AIDS & Sex Transmitted Virus Vaccines, Beijing, Peoples R China
[2] WHO Collaborating Ctr Standardizat & Evaluat Biol, Beijing, Peoples R China
[3] Wuhan Inst Biol Prod Co Ltd, Wuhan, Peoples R China
[4] Chinese Acad Med Sci & Peking Union Med Coll, Suzhou Inst Syst Med, State Key Lab Common Mech Res Major Dis, Suzhou, Jiangsu, Peoples R China
[5] Guangzhou Natl Lab, Guangzhou, Peoples R China
[6] Beijing Sciecure Pharmaceut Co Ltd, Dept Res & Dev, Beijing, Peoples R China
[7] Changping Lab, Beijing, Peoples R China
[8] Peking Union Med Coll, Grad Sch, Beijing, Peoples R China
来源：
FRONTIERS IN MICROBIOLOGY | 2024年 / 15卷
关键词：
multi-epitope; vaccine; HEV; ORF2; ORF3; CAPSID PROTEIN; PREDICTION; IDENTIFICATION; HEV; INFECTION; RELEASE; SERUM; WEB;
D O I：
10.3389/fmicb.2024.1372069
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Introduction Hepatitis E virus (HEV), with heightened virulence in immunocompromised individuals and pregnant women, is a pervasive threat in developing countries. A globaly available vaccine against HEV is currently lacking.Methods We designed a multi-epitope vaccine based on protein ORF2 and ORF3 of HEV using immunoinformatics.Results The vaccine comprised 23 nontoxic, nonallergenic, soluble peptides. The stability of the docked peptide vaccine-TLR3 complex was validated by molecular dynamic simulations. The induction of effective cellular and humoral immune responses by the multi-peptide vaccine was verified by simulated immunization.Discussion These findings provide a foundation for future HEV vaccine studies.
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页数：10
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