Multi-epitope vaccine design for hepatitis E virus based on protein ORF2 and ORF3

Introduction Hepatitis E virus (HEV), with heightened virulence in immunocompromised individuals and pregnant women, is a pervasive threat in developing countries. A globaly available vaccine against HEV is currently lacking.Methods We designed a multi-epitope vaccine based on protein ORF2 and ORF3 of HEV using immunoinformatics.Results The vaccine comprised 23 nontoxic, nonallergenic, soluble peptides. The stability of the docked peptide vaccine-TLR3 complex was validated by molecular dynamic simulations. The induction of effective cellular and humoral immune responses by the multi-peptide vaccine was verified by simulated immunization.Discussion These findings provide a foundation for future HEV vaccine studies.