Multi-epitope vaccine design for hepatitis E virus based on protein ORF2 and ORF3

被引：3

作者：

Lu, Qiong ^{[1
,2
]}

Wu, Hao ^{[1
,2
,3
]}

Meng, Jing ^{[4
]}

Wang, Jiangyuan ^{[5
]}

Wu, Jiajing ^{[6
]}

Liu, Shuo ^{[7
,8
]}

Tong, Jincheng ^{[1
,2
]}

Nie, Jianhui ^{[1
,2
]}

Huang, Weijin ^{[1
,2
]}

机构：

[1] Natl Inst Food & Drug Control, Inst Biol Prod Control, Div HIV AIDS & Sex Transmitted Virus Vaccines, Beijing, Peoples R China

[2] WHO Collaborating Ctr Standardizat & Evaluat Biol, Beijing, Peoples R China

[3] Wuhan Inst Biol Prod Co Ltd, Wuhan, Peoples R China

[4] Chinese Acad Med Sci & Peking Union Med Coll, Suzhou Inst Syst Med, State Key Lab Common Mech Res Major Dis, Suzhou, Jiangsu, Peoples R China

[5] Guangzhou Natl Lab, Guangzhou, Peoples R China

[6] Beijing Sciecure Pharmaceut Co Ltd, Dept Res & Dev, Beijing, Peoples R China

[7] Changping Lab, Beijing, Peoples R China

[8] Peking Union Med Coll, Grad Sch, Beijing, Peoples R China

来源：

FRONTIERS IN MICROBIOLOGY | 2024年 / 15卷

关键词：

multi-epitope; vaccine; HEV; ORF2; ORF3; CAPSID PROTEIN; PREDICTION; IDENTIFICATION; HEV; INFECTION; RELEASE; SERUM; WEB;

D O I：

10.3389/fmicb.2024.1372069

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Introduction Hepatitis E virus (HEV), with heightened virulence in immunocompromised individuals and pregnant women, is a pervasive threat in developing countries. A globaly available vaccine against HEV is currently lacking.Methods We designed a multi-epitope vaccine based on protein ORF2 and ORF3 of HEV using immunoinformatics.Results The vaccine comprised 23 nontoxic, nonallergenic, soluble peptides. The stability of the docked peptide vaccine-TLR3 complex was validated by molecular dynamic simulations. The induction of effective cellular and humoral immune responses by the multi-peptide vaccine was verified by simulated immunization.Discussion These findings provide a foundation for future HEV vaccine studies.

引用

页数：10

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