In this paper, three Lupinus albus L. and two L. angustifolius L. genotypes were examined and compared in terms of volatiles or semi volatiles using GC-MS and SPME techniques. For this aim, the seeds of lupins were ground using a hand grinder, and this powder raw material was used to determine the content. Approximately 102 compounds obtained from lupin genotypes were identified for the first time, which mainly included benzene-methyl (1-methyl ethyl), 5-Allyl-4-(1-(p-amino phenyl) ethylidenehyl, 2-propen-1-ol, 3-phenyl-, m-Mentha-4,8,diene-(1S,3S)-(+), gamma terpinene. Thee compounds were determined to exist at different rates in different. As a result, it becomes evident that such studies aiming at revealing the active matters from plants or plant parts and their possible potentials, are of great importance in terms of more specific studies to be carried out in the future.