Detection of classical BSE prions in asymptomatic cows after inoculation with atypical/Nor98 scrapie

被引：7

作者：

Betancor, Marina ^{[1
]}

Marin, Belen ^{[1
]}

Otero, Alicia ^{[1
]}

Hedman, Carlos ^{[1
]}

Romero, Antonio ^{[2
]}

Barrio, Tomas ^{[3
]}

Sevilla, Eloisa ^{[1
]}

Douet, Jean-Yves ^{[3
]}

Huor, Alvina ^{[3
]}

Badiola, Juan Jose ^{[1
]}

Andreoletti, Olivier ^{[3
]}

Bolea, Rosa ^{[1
]}

机构：

[1] Univ Zaragoza, Fac Vet, Ctr Encefalopatias & Enfermedades Transmisibles Em, Inst Agroalimentario Aragon IA2, Zaragoza 50013, Spain

[2] Univ Zaragoza, Hosp Vet, Serv Cirugia & Med Equina, Zaragoza 50013, Spain

[3] Ecole Natl Vet Toulouse, UMR INRAE ENVT Interact Hotes Agents Pathogenes 12, F-31076 Toulouse, France

来源：

VETERINARY RESEARCH | 2023年 / 54卷 / 01期

关键词：

Prion; PrPSc; atypical scrapie; bovine spongiform encephalopathy; INFECTIVITY; VARIANT; SHEEP;

D O I：

10.1186/s13567-023-01225-2

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The emergence of bovine spongiform encephalopathy (BSE) prions from atypical scrapie has been recently observed upon experimental transmission to rodent and swine models. This study aimed to assess whether the inoculation of atypical scrapie could induce BSE-like disease in cattle. Four calves were intracerebrally challenged with atypical scrapie. Animals were euthanized without clinical signs of prion disease and tested negative for PrPSc accumulation by immunohistochemistry and western blotting. However, an emergence of BSE-like prion seeding activity was detected during in vitro propagation of brain samples from the inoculated animals. These findings suggest that atypical scrapie may represent a potential source of BSE infection in cattle.

引用

页数：7

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