Proteomic profiling of small extracellular vesicles derived from mouse pancreatic cancer and stellate cells: Role in pancreatic cancer

被引：1

作者：

Perera, Chamini J. ^{[1
,2
]}

Hosen, S. M. Zahid ^{[1
,2
]}

Khan, Tanzila ^{[1
,2
]}

Fang, Haoyun ^{[3
,4
,5
,6
]}

Mekapogu, Alpha Raj ^{[1
,2
]}

Xu, Zhihong ^{[1
,2
]}

Falasca, Marco ^{[7
]}

Chari, Suresh T. ^{[8
]}

Wilson, Jeremy S. ^{[1
,2
]}

Pirola, Ron ^{[1
,2
]}

Greening, David W. ^{[3
,4
,5
,6
]}

Apte, Minoti V. ^{[1
,2
,9
]}

机构：

[1] UNSW Sydney, Fac Med & Hlth, Sch Clin Med, Pancreat Res Grp, South West Sydney Clin Campus, Sydney, Australia

[2] Ingham Inst Appl Med Res, Liverpool, NSW, Australia

[3] La Trobe Univ, Res Ctr Extracellular Vesicles, Bundoora, Australia

[4] Baker Heart & Diabet Inst, Melbourne, Australia

[5] La Trobe Univ, Sch Agr Biomed & Environm, Dept Cardiovasc Res Translat & Implementat CaRTI, Bundoora, Australia

[6] Univ Melbourne, Dept Cardiometab Hlth, Melbourne, Australia

[7] Curtin Univ, Fac Hlth Sci, Metab Signalling Grp, Curtin Med Sch, Perth, Australia

[8] Univ Texas MD Anderson Canc Ctr, Dept Gastroenterol Hepatol & Nutr, Houston, TX USA

[9] UNSW, Ingham Inst Appl Med Res, Pancreat Res Grp, South Western Sydney Clin Campus,Level 4, Liverpool, NSW 2170, Australia

来源：

PROTEOMICS | 2024年 / 24卷 / 11期

基金：

澳大利亚国家健康与医学研究理事会;

关键词：

biomarkers; pancreatic cancer; pancreatic stellate cells; proteomics; small extra cellular vesicles; EXPRESSION; EXOSOMES; GENE; IDENTIFICATION; PROTEINS; PLATFORM; RECEPTOR; PERSEUS; CA19-9;

D O I：

10.1002/pmic.202300067

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Small extracellular vesicles (sEVs) are cell-derived vesicles evolving as important elements involved in all stages of cancers. sEVs bear unique protein signatures that may serve as biomarkers. Pancreatic cancer (PC) records a very poor survival rate owing to its late diagnosis and several cancer cell-derived proteins have been reported as candidate biomarkers. However, given the pivotal role played by stellate cells (PSCs, which produce the collagenous stroma in PC), it is essential to also assess PSC-sEV cargo in biomarker discovery. Thus, this study aimed to isolate and characterise sEVs from mouse PC cells and PSCs cultured alone or as co-cultures and performed proteomic profiling and pathway analysis. Proteomics confirmed the enrichment of specific markers in the sEVs compared to their cells of origin as well as the proteins that are known to express in each of the culture types. Most importantly, for the first time it was revealed that PSC-sEVs are enriched in proteins (including G6PI, PGAM1, ENO1, ENO3, and LDHA) that mediate pathways related to development of diabetes, such as glucose metabolism and gluconeogenesis revealing a potential role of PSCs in pancreatic cancer-related diabetes (PCRD). PCRD is now considered a harbinger of PC and further research will enable to identify the role of these components in PCRD and may develop as novel candidate biomarkers of PC.

引用

页数：17

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