Pharmacological evaluation of a traditional Brazilian medicinal plant, Monteverdia ilicifolia. Part I- Preclinical safety study

被引：1

作者：

Danilevicz, Chris Krebs ^{[1
]}

Pizzolato, Lolita Schneider ^{[2
]}

Bianchi, Sara Elis ^{[2
,3
]}

Meirelles, Gabriela ^{[2
]}

Bassani, Valquiria Linck ^{[3
,4
]}

Siqueira, Ionara Rodrigues ^{[1
,2
,5
]}

机构：

[1] Univ Fed Rio Grande do Sul, Programa Posgrad Ciencias Biol Farmacol & Terapeut, Porto Alegre, RS, Brazil

[2] Univ Fed Rio Grande do Sul, Dept Farmacol, Inst Ciencias Basicas Saude, Porto Alegre, RS, Brazil

[3] Univ Fed Rio Grande do Sul, Fac Farm, Lab Desenvolvimento Galenico, Porto Alegre, RS, Brazil

[4] Univ Fed Rio Grande do Sul, Programa Posgrad Ciencias Farmaceut, Porto Alegre, RS, Brazil

[5] Univ Fed Rio Grande do Sul, Dept Farmacol, Inst Ciencias Basicas Saude, Rua Sarmento Leite 500,sala 327, BR-90050170 Porto Alegre, RS, Brazil

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2024年 / 324卷

关键词：

Maytenus ilicifolia; Herbal medicine; Medicinal plant; Drug interaction; Liver; Intestine; Cell injury; Cytochrome P450; P-GLYCOPROTEIN; INHIBITION; LIVER; CYP3A4; BIOAVAILABILITY; QUERCETIN; VITRO; 3A4;

D O I：

10.1016/j.jep.2024.117806

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Ethnopharmacological relevance: Monteverdia ilicifolia (Maytenus ilicifolia, Celastraceae), known as "espinheirasanta", has been widely used in Brazil to manage mainly gastrointestinal diseases. This species has been listed in the Brazilian Pharmacopeia and in the National List of Essential Medicines (RENAME). Considering that clinical studies about M. ilicifolia are rare, our group has been performing a broader project designed to evaluate the efficacy of M. ilicifolia capsules in a clinical trial, for this reason, approaches to provide safety to those patients are relevant. Aim of the study: We aimed to investigate the potential pharmacokinetic interaction and hepatotoxicity and intestinal toxicity of an aqueous extract of M. ilicifolia and its main phytocompounds, catequin, epicatequin, and quercetin. Methods and materials: Slices of liver and intestine of Wistar rats were incubated with different concentrations of M. ilicifolia extract or isolated compounds (catechin, epicatechin and quercetin). Commercial kits were used to evaluate enzyme activities of CYP2D6 and CYP3A4 isoforms, as well as cell viability (MTT) assay and intracellular enzymes leakage, specifically lactate dehydrogenase (LDH), alkaline phosphatase (AP), aspartate aminotransferase (AST), alanine aminotransferase (ALT) were studied. Results: Incubation with M. ilicifolia extract, catechin, epicatechin and quercetin did not affect significantly any evaluated parameter in intestines. The intracellular enzymes leakages, CYP2D6, LDH and AST, were increased with M. ilicifolia extract and quercetin in liver slices. Conclusions: Our in vitro findings highlighted, for the first time, the potential hepatotoxicity induced by an aqueous extract of M. ilicifolia, consequently this species and its products should be avoided in liver diseases, supporting that studies of safety must be performed including in the context of traditional medicinal plants.

引用

页数：6

共 29 条

[21] Liver biopsy in the evaluation of patients with chronic hepatitis C who have repeatedly normal or near-normal serum alanine aminotransferase levels [J].