Evaluation of cigarette smoke-induced oxidative stress and inflammation in BEAS-2B cells based on a lung microfluidic chip

被引:8
|
作者
Xue, Jingxian [1 ]
Li, Zezhi [1 ,2 ]
Li, Xiang [1 ]
Hua, Chenfeng [1 ]
Shang, Pingping [1 ]
Zhao, Junwei [1 ]
Liu, Kejian [1 ]
Xie, Fuwei [1 ]
机构
[1] Zhengzhou Tobacco Res Inst CNTC, Key Lab Tobacco Chem, 2 Fengyang St, Zhengzhou 450001, Peoples R China
[2] Beijing Technol & Business Univ, Beijing 100048, Peoples R China
关键词
Microfluidic chip; Cigarette smoke; Reactive oxygen species; Inflammation; Gaseous pollutants; AIR-POLLUTION; EXPOSURE; RESPONSES; RELEASE;
D O I
10.1016/j.fct.2023.113787
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Oxidative stress and inflammation induced by cigarette smoking are associated with the pathology process of various chronic respiratory diseases, including asthma, emphysema, chronic obstructive pulmonary disease and cancer. Compared with conventional cell culture techniques, microfluidic chips can provide a continuous nutrient supply, mimic the in vivo physiological microenvironment of the cells, and conduct an integrated and flexible analysis of cell status and functions. Here, we designed and fabricated a bionic-lung chip, which was applied to perform cigarette smoke exposure of BEAS-2B cells cultured at the gas-liquid interface. The oxidative stress and inflammation in the cells exposed to cigarette smoke were investigated on chip. The results showed that cellular damage, oxidative stress and inflammatory response induced by cigarette smoke in the chip were dependent on smoke concentration and time after smoke exposure. N-Acetylcysteine (NAC) significantly inhibited these effects of cigarette smoke exposure on the cells at the gas-liquid interface within the chip.
引用
收藏
页数:10
相关论文
共 50 条
  • [1] Evaluation of whole cigarette smoke induced oxidative stress in A549 and BEAS-2B cells
    Zhang, Shimin
    Li, Xiang
    Xie, Fuwei
    Liu, Kejian
    Liu, Huimin
    Xie, Jianping
    ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 2017, 54 : 40 - 47
  • [2] Combining a lung microfluidic chip exposure model with transcriptomic analysis to evaluate the inflammation in BEAS-2B cells exposed to cigarette smoke
    Li, Zezhi
    Li, Xiang
    Feng, Boyang
    Xue, Jingxian
    Zhao, Junwei
    Zhu, Qingqing
    Liu, Kejian
    Xie, Fuwei
    Xie, Jianping
    ANALYTICA CHIMICA ACTA, 2024, 1287
  • [3] Cigarette smoke triggers inflammation mediated by autophagy in BEAS-2B cells
    Xu, Liangtao
    Li, Xiang
    Wang, Huiting
    Xie, Fuwei
    Liu, Huimin
    Xie, Jianping
    ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2019, 184
  • [4] Calpeptin attenuates cigarette smoke-induced pulmonary inflammation via suppressing calpain/IκBα signaling in mice and BEAS-2B cells
    Zuo, Jingjing
    Hu, Zhangwei
    Liu, Tao
    Chen, Chen
    Tao, Zezhang
    Chen, Shiming
    Li, Fen
    PATHOLOGY RESEARCH AND PRACTICE, 2018, 214 (08) : 1199 - 1209
  • [5] RBM5 Inhibits the Proliferation of Cigarette Smoke-Induced Cancerous Transformation of BEAS-2B Cells
    Zhang Jie
    Lv Xuejiao
    Hao Yuqiu
    Su Zhenzhong
    Meng Guangping
    Wang Qi
    Zhang Lin
    CHEST, 2016, 149 (04) : 257A - 257A
  • [6] Ebeiedinone and peimisine inhibit cigarette smoke extract-induced oxidative stress injury and apoptosis in BEAS-2B cells
    Liu, Chuanlan
    Zhu, Xiaomu
    Aga, Erbu
    Tse, Wai Ming
    Tse, Kathy Wai Gaun
    Liu, Yanyong
    Ye, Bengui
    CELL STRESS & CHAPERONES, 2024, 29 (06): : 697 - 708
  • [7] RBM5 regulates EGFR involving in cancerous transformation of cigarette smoke-induced BEAS-2B cells
    Zhang, Jie
    Lv, Xuejiao
    Hao, Yuqiu
    Su, Zhenzhong
    Li, Junyao
    Du, Yanwei
    EUROPEAN RESPIRATORY JOURNAL, 2015, 46
  • [8] Aberrant DNA methylation in radon and/or cigarette smoke-induced malignant transformation in BEAS-2B human lung cell line
    Huang, Huanhuan
    Ji, Yahui
    Zhang, Jiayu
    Su, Zhigang
    Liu, Mingxing
    Tong, Jian
    Ge, Cuicui
    Chen, Tao
    Li, Jianxiang
    JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, 2017, 80 (23-24): : 1321 - 1330
  • [9] Eucalyptol attenuates cigarette smoke-induced acute lung inflammation and oxidative stress in the mouse
    Kennedy-Feitosa, Emanuel
    Okuro, Renata Tiemi
    Ribeiro, Vanessa Pinho
    Lanzetti, Manuella
    Barroso, Marina Valente
    Zin, Walter Araujo
    Porto, Luis Cristovao
    Brito-Gitirana, Lycia
    Valenca, Samuel Santos
    PULMONARY PHARMACOLOGY & THERAPEUTICS, 2016, 41 : 11 - 18
  • [10] Targeting Oxidative Stress Reduces Cigarette Smoke-Induced Lung Inflammation And Wasting In Mice
    Vlahos, R.
    Bernardo, I.
    Passey, S.
    Seow, H. J.
    Yatmaz, S.
    Selemidis, S.
    Hansen, M. J.
    Bozinovski, S.
    AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2015, 191