SARS-CoV-2 RNA-dependent RNA polymerase as a target for high-throughput drug screening

被引：5
作者：
Wu, Jiahui [1 ]
Chen, Zhiqiang [1 ,2 ]
Han, Xue [3 ]
Chen, Qiaoqiao [1 ]
Wang, Yintao [1 ]
Feng, Tingting [1 ]
机构：
[1] Soochow Univ, Inst Biol & Med Sci, Jiangsu Key Lab Infect & Immun, Suzhou 215123, Jiangsu, Peoples R China
[2] Soochow Univ, Affiliated Hosp 1, Dept Nucl Med, Suzhou 215000, Jiangsu, Peoples R China
[3] Qingdao Univ, Affiliated Hosp, Dept Clin Lab, 59 Haier Rd, Qingdao 266000, Shandong, Peoples R China
来源：
FUTURE VIROLOGY | 2023年
关键词：
COVID-19; high-throughput screening; nsp12-nsp8-nsp7; RNA-dependent RNA polymerase; SARS-CoV-2; STRUCTURAL BASIS; REPLICATION; CORONAVIRUS; SARS; PROTEIN; IDENTIFICATION; NUCLEOSIDE; INHIBITORS; ANALOGS; NSP12;
D O I：
10.2217/fvl-2021-0335
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The ongoing COVID-19 pandemic caused by the SARS-CoV-2 has necessitated rapid development of drug screening tools. RNA-dependent RNA polymerase (RdRp) is a promising target due to its essential functions in replication and transcription of viral genome. To date, through minimal RNA synthesizing machinery established from cryo-electron microscopy structural data, there has been development of high-throughput screening assays for directly screening inhibitors that target the SARS-CoV-2 RdRp. Here, we analyze and present verified techniques that could be used to discover potential anti-RdRp agents or repurposing of approved drugs to target the SARS-CoV-2 RdRp. In addition, we highlight the characteristics and application value of cell-free or cell-based assays in drug discovery.
引用
收藏
页码：51 / 62
页数：12
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