SARS-CoV-2 RNA-dependent RNA polymerase as a target for high-throughput drug screening

被引：8

作者：

Wu, Jiahui ^{[1
]}

Chen, Zhiqiang ^{[1
,2
]}

Han, Xue ^{[3
]}

Chen, Qiaoqiao ^{[1
]}

Wang, Yintao ^{[1
]}

Feng, Tingting ^{[1
]}

机构：

[1] Soochow Univ, Inst Biol & Med Sci, Jiangsu Key Lab Infect & Immun, Suzhou 215123, Jiangsu, Peoples R China

[2] Soochow Univ, Affiliated Hosp 1, Dept Nucl Med, Suzhou 215000, Jiangsu, Peoples R China

[3] Qingdao Univ, Affiliated Hosp, Dept Clin Lab, 59 Haier Rd, Qingdao 266000, Shandong, Peoples R China

来源：

FUTURE VIROLOGY | 2023年

关键词：

COVID-19; high-throughput screening; nsp12-nsp8-nsp7; RNA-dependent RNA polymerase; SARS-CoV-2; STRUCTURAL BASIS; REPLICATION; CORONAVIRUS; SARS; PROTEIN; IDENTIFICATION; NUCLEOSIDE; INHIBITORS; ANALOGS; NSP12;

D O I：

10.2217/fvl-2021-0335

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The ongoing COVID-19 pandemic caused by the SARS-CoV-2 has necessitated rapid development of drug screening tools. RNA-dependent RNA polymerase (RdRp) is a promising target due to its essential functions in replication and transcription of viral genome. To date, through minimal RNA synthesizing machinery established from cryo-electron microscopy structural data, there has been development of high-throughput screening assays for directly screening inhibitors that target the SARS-CoV-2 RdRp. Here, we analyze and present verified techniques that could be used to discover potential anti-RdRp agents or repurposing of approved drugs to target the SARS-CoV-2 RdRp. In addition, we highlight the characteristics and application value of cell-free or cell-based assays in drug discovery.

引用

页码：51 / 62

页数：12

共 78 条

[21] From SARS to MERS: 10 years of research on highly pathogenic human coronaviruses [J].