Engineered living materials grown from programmable Aspergillus niger mycelial pellets

被引:12
|
作者
Li, Ke [1 ]
Wei, Zhen [2 ]
Jia, Jianyao [1 ]
Xu, Qing [1 ]
Liu, Hao [2 ,3 ]
Zhong, Chao [4 ,5 ]
Huang, He [1 ]
机构
[1] Nanjing Normal Univ, Sch Food Sci & Pharmaceut Engn, Nanjing 211816, Peoples R China
[2] Tianjin Univ Sci & Technol, Coll Biotechnol, MOE Key Lab Ind Fermentat Microbiol, Tianjin 300457, Peoples R China
[3] Tianjin Univ Sci & Technol, Tianjin Engn Res Ctr Microbial Metab & Fermentat P, Tianjin 300457, Peoples R China
[4] Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Ctr Mat Synthet Biol, Shenzhen 518055, Peoples R China
[5] Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, CAS Key Lab Quantitat Engn Biol, Shenzhen 518055, Peoples R China
基金
国家重点研发计划;
关键词
Engineered living material; Filamentous fungus; Aspergillus niger; Genetic circuit; Melanin;
D O I
10.1016/j.mtbio.2023.100545
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The development of engineered living materials (ELMs) has recently attracted significant attention from re-searchers across multiple disciplines. Fungi-derived ELMs represent a new type of macroscale, cost-effective, environmentally sustainable materials. However, current fungi-based ELMs either have to undergo a final pro-cess to heat-kill the living cells or rely on the co-culture with a model organism for functional modification, which hinders the engineerability and versatility of these materials. In this study, we report a new type of ELMs - grown from programmable Aspergillus niger mycelial pellets - by a simple filtration step under ambient conditions. We demonstrate that A. Niger pellets can provide sufficient cohesion to maintain large-area self-supporting structures even under low pH conditions. Subsequently, by tuning the inducible expression of genes involved in melanin biosynthesis, we verified the fabrication of self-supporting living membrane materials with tunable colors in response to xylose concentration in the surroundings, which can be further explored as a potential biosensor for detecting xylose level in industrial wastewater. Notably, the living materials remain alive, self-regenerative, and functional even after 3-month storage. Thus, beyond reporting a new engineerable fungi chassis for constructing ELMs, our study provides new opportunities for developing bulk living materials for real-world applications such as the production of fabrics, packaging materials, and biosensors.
引用
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页数:8
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