Markedly Different Effects of Monovalent Cations on the Efficiency of Gene Expression

被引：0
作者：
Nishio, Takashi [1 ,2 ]
Masaoka, Tomoya [1 ]
Yoshikawa, Yuko [1 ]
Sadakane, Koichiro [1 ]
Kenmotsu, Takahiro [1 ]
Schiessel, Helmut [2 ]
Yoshikawa, Kenichi [1 ,3 ]
机构：
[1] Doshisha Univ, Fac Life & Med Sci, Kyoto 6100394, Japan
[2] Tech Univ Dresden, Cluster Excellence Phys Life, D-01307 Dresden, Germany
[3] Kyoto Univ, Inst Adv Study, Ctr Integrat Med & Phys, Kyoto 6068501, Japan
来源：
ADVANCED BIOLOGY | 2023年 / 7卷 / 03期
关键词：
cell-free gene expression; luciferase assay; monovalent cations; transcription-translation; HOFMEISTER SERIES; COUNTERION-CONDENSATION; DNA COMPACTION; NA+; IONS; K+; HYDRATION; BINDING; TRANSLATION; SELECTIVITY;
D O I：
10.1002/adbi.202200164
中图分类号：
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号：
0805 ; 080501 ; 080502 ;
摘要：
The effect of monovalent cations on a cell-free transcription-translation (TX-TL) system is examined using a luciferase assay. It is found that the potency for all ions analyzed here is in the order Rb+ > K+ > Cs+ > Na+ approximate to Li+ > (CH3)(4)N+, where Rb+ is most efficient at promoting TX-TL and the ions of Li+, Na+, and (CH3)(4)N+ exhibit an inhibitory effect. Similar promotion/inhibition effects are observed for cell-free TL alone with an mRNA template.
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页数：8
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  • [1] NTP concentration switches transcriptional activity by changing the large-scale structure of DNA
    Akitaya, T
    Tsumoto, K
    Yamada, A
    Makita, N
    Kubo, K
    Yoshikawa, K
    [J]. BIOMACROMOLECULES, 2003, 4 (05) : 1121 - 1125
  • [2] Chromatin compaction under mixed salt conditions: Opposite effects of sodium and potassium ions on nucleosome array folding
    Allahverdi, Abdollah
    Chen, Qinming
    Korolev, Nikolay
    Nordenskioeld, Lars
    [J]. SCIENTIFIC REPORTS, 2015, 5
  • [3] Auffinger P, 2016, METAL IONS LIFE SCI, V16, P167, DOI 10.1007/978-3-319-21756-7_6
  • [4] RELATIVE BINDING AFFINITIES OF MONO-VALENT CATIONS FOR DOUBLE-STRANDED DNA
    BLEAM, ML
    ANDERSON, CF
    RECORD, MT
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-PHYSICAL SCIENCES, 1980, 77 (06): : 3085 - 3089
  • [5] A Molecular Model for Lithium's Bioactive Form
    Briggs, Katharine T.
    Giulian, Gary G.
    Li, Gong
    Kao, Joseph P. Y.
    Marino, John P.
    [J]. BIOPHYSICAL JOURNAL, 2016, 111 (02) : 294 - 300
  • [6] The Hofmeister series: salt and solvent effects on interfacial phenomena
    Cacace, MG
    Landau, EM
    Ramsden, JJ
    [J]. QUARTERLY REVIEWS OF BIOPHYSICS, 1997, 30 (03) : 241 - 277
  • [7] CAHN F, 1978, J BIOL CHEM, V253, P7798
  • [8] Ions from the Hofmeister series and osmolytes: effects on proteins in solution and in the crystallization process
    Collins, KD
    [J]. METHODS, 2004, 34 (03) : 300 - 311
  • [9] A compact RNA tertiary structure contains a buried Backbone-K+ complex
    Conn, GL
    Gittis, AG
    Lattman, EE
    Misra, VK
    Draper, DE
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 2002, 318 (04) : 963 - 973
  • [10] Na+ and K+ Allosterically Regulate Cooperative DNA Binding by the Human Progesterone Receptor
    Connaghan, Keith D.
    Heneghan, Aaron F.
    Miura, Michael T.
    Bain, David L.
    [J]. BIOCHEMISTRY, 2010, 49 (03) : 422 - 431
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