Surfactant-assisted crystallization of lysozyme and its mechanism

被引:1
作者
Song, Yujie [1 ]
Ren, Rongfei [1 ]
Su, Min [1 ]
Liang, Zhihan [1 ]
Hao, Qi [1 ]
机构
[1] Hebei Univ Technol, Sch Chem Engn, Tianjin 300130, Peoples R China
来源
PARTICUOLOGY | 2024年 / 90卷
关键词
Protein; Crystallization; Surfactant; Lysozyme; Molecular interaction; Mechanism; EGG-WHITE; PROTEIN; BINDING; PRECIPITATION; STABILITY; PH;
D O I
10.1016/j.partic.2023.11.025
中图分类号
TQ [化学工业];
学科分类号
0817 ;
摘要
Surfactants were used to assist in the crystallization of lysozyme. Three types of surfactants with different structures (sodium dodecyl sulfate, cetyltrimethylammonium bromide, or betaine hydrochloride [BH]) were used. The study indicated that three surfactants were all capable of shortening the crystallization induction time of lysozyme, with BH inducing the shortest crystallization induction time (t 1/4 1 min) and the largest crystal size (37 mm). The molecular interactions among each of the three surfactants aforementioned and lysozyme were also investigated by simulations. It was found that hydrogen bonding dominantly acted between the carboxyl structure of the surfactant BH and the lysozyme, which produced the most favorable conformation for the self-assembly of lysozyme (promote lysozyme orderly aggregation and shorten the crystallization induction time). On this basis, the mechanism of surfactant-promoted lysozyme crystallization was proposed. (c) 2024 Chinese Society of Particuology and Institute of Process Engineering, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:199 / 208
页数:10
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