Investigation of roles of IL-8 (+781 C/T) and MMP-2 (-735 C/T) gene variations in early diagnosis of bladder cancer and progression

被引：2
作者：
Alkanli, Nevra [1 ]
Ay, Arzu [2 ]
Cevik, Gokhan [3 ]
机构：
[1] Halic Univ, Fac Med, Dept Biophys, Istanbul, Turkey
[2] Trakya Univ, Fac Med, Dept Biophys, Edirne, Turkey
[3] Trakya Univ, Fac Med, Dept Urol, Edirne, Turkey
来源：
MOLECULAR BIOLOGY REPORTS | 2023年 / 50卷 / 01期
关键词：
Bladder cancer; Matrix metalloproteinase; Interleukin; Gene variations; PCR-RFLP; ONCOLOGIC OUTCOMES; LUNG-CANCER; POLYMORPHISMS; RISK; SUSCEPTIBILITY; INTERLEUKIN-8; ASSOCIATION; IL-8-251A/T;
D O I：
10.1007/s11033-022-07881-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Background The aim of our study is to investigate the roles of IL-8 (+ 781 C/T) and MMP-2 (-735 C/T) gene variations in early diagnosis and progression of BCA. Methods Polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) methods were used to determine the genotype distributions of IL-8 (+ 781 C/T) and MMP-2 (-735 C/T) gene variations. Results In our study, the genotype distributions of IL-8 (+ 781 C/T) and MMP-2 (-735 C/T) gene variations were not found to be significantly different between the patient and control groups. In addition, C and T allele frequencies for these gene variations were not different from the Hardy-Weinberg distribution in patient and control groups. However, when the combined genotype analyzes for these gene variations were evaluated, CC-CC and CT-CC combined genotypes for + 781 C/T / -735 C/T gene variations were observed significantly more in the patient group compared to other genotypes. Conclusion Although IL-8 (+ 781 C/T) and MMP-2 (-735 C/T) gene variations were not found to be genetic risk factors in the Thrace population in our study, CC-CC and CT-CC combined genotypes were determined as genetic risk factors for BCA susceptibility. The combined genotypes obtained as a result of the combined genotype analysis of these genetic variations that are effective in tumor progression may be considered to be important biomarkers for the early diagnosis and progression of BCA.
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页码：443 / 451
页数：9
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