Insertion of unnatural metal ligand in the heme pocket of nitrophorin through protein semi-synthesis: Toward biomimicking binuclear active sites

被引：3

作者：

Chen, Yonglu ^{[1
]}

Zhou, Yi ^{[1
]}

He, Chunmao ^{[1
]}

机构：

[1] South China Univ Technol, Sch Chem & Chem Engn, Guangzhou 510640, Peoples R China

来源：

CHINESE CHEMICAL LETTERS | 2023年 / 34卷 / 12期

基金：

中国国家自然科学基金;

关键词：

Protein semi-synthesis; Protein modification; Nitrophorin; 2; Heteronuclear active site; Native chemical ligation; NITRIC-OXIDE; COMPLEX; BINDING; MODELS; OXYGEN;

D O I：

10.1016/j.cclet.2023.108251

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Herein, we report a semi-synthetic strategy affording a nitrophorin 2 (NP2) variant with a N,N -bis(2-pyridylmethyl)amine (Dpa) ligand as sidechain selectively installed at position 27, which was assembled from a synthetic peptide thioester bearing the Dpa ligand and an expressed protein segment via native chemical ligation. The semi-synthetic NP2 was able to accept the natural heme b cofactor and the Dpa ligand was able to bind Cu(II)/Fe(III) ions, leading to heteronuclear active site.(c) 2023 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.

引用

页数：4