Volume EM: a quiet revolution takes shape

被引：32

作者：

Collinson, Lucy M. ^{[1
]}

Bosch, Carles ^{[2
]}

Bullen, Anwen ^{[3
]}

Burden, Jemima J. ^{[4
]}

Carzaniga, Raffaella ^{[5
]}

Cheng, Cheng ^{[6
]}

Darrow, Michele C. ^{[7
,8
]}

Fletcher, Georgina ^{[9
]}

Johnson, Errin ^{[10
]}

Narayan, Kedar ^{[11
,12
]}

Peddie, Christopher J. ^{[1
]}

Winn, Martyn ^{[13
]}

Wood, Charles ^{[14
]}

Patwardhan, Ardan ^{[15
]}

Kleywegt, Gerard J. ^{[15
]}

Verkade, Paul ^{[16
]}

机构：

[1] Francis Crick Inst, Electron Microscopy Sci Technol Platform, London, England

[2] Francis Crick Inst, Sensory Circuits & Neurotechnol Lab, London, England

[3] UCL, UCL Ear Inst, London, England

[4] UCL, Lab Mol Cell Biol, London, England

[5] Carl Zeiss Ltd, Zeiss Grp, Zeiss Res Microscopy Solut, Camborne, England

[6] ConnectomX Ltd, Oxford, England

[7] Rosalind Franklin Inst, Artificial Intelligence & Informat, Didcot, England

[8] SPT Labtech Ltd, Melbourn, England

[9] BioImagingUK, Oxford, England

[10] Univ Oxford, Dunn Sch Bioimaging Facil, Sir William Dunn Sch Pathol, Oxford, England

[11] NCI, NIH, Ctr Mol Microscopy, Ctr Canc Res, Bethesda, MD USA

[12] Frederick Natl Lab Canc Res, Canc Res Technol Program, Frederick, MD USA

[13] Rutherford Appleton Lab, UKRI STFC, Didcot, England

[14] Univ Portsmouth, Future Technol Ctr, Sch Mech & Design Engn, Portsmouth, England

[15] European Bioinformat Inst EMBL EBI, European Mol Biol Lab, Hinxton, England

[16] Univ Bristol, Sch Biochem, Bristol, England

来源：

NATURE METHODS | 2023年 / 20卷 / 06期

基金：

英国医学研究理事会; 英国惠康基金; 英国生物技术与生命科学研究理事会; 美国国家卫生研究院;

关键词：

ELECTRON-MICROSCOPY; NERVOUS-SYSTEM;

D O I：

10.1038/s41592-023-01861-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Volume electron microscopy (vEM) is a group of techniques that reveal the 3D ultrastructure of cells and tissues through continuous depths of at least 1 micrometer. A burgeoning grassroots community effort is fast building the profile and revealing the impact of vEM technology in the life sciences and clinical research.

引用

页码：777 / 782

页数：6

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