Dissolving microneedle system containing Ag nanoparticle-decorated silk fibroin microspheres and antibiotics for synergistic therapy of bacterial biofilm infection

被引：8

作者：

Li, Yao ^{[1
]}

Gong, Jue-Ying ^{[1
]}

Wang, Po ^{[1
]}

Fu, Han ^{[1
]}

Yousef, Faraj ^{[2
]}

Xie, Rui ^{[1
]}

Wang, Wei ^{[1
]}

Liu, Zhuang ^{[1
]}

Pan, Da -Wei ^{[1
]}

Ju, Xiao-Jie ^{[1
]}

Chu, Liang-Yin ^{[1
]}

机构：

[1] Sichuan Univ, Sch Chem Engn, Chengdu 610065, Peoples R China

[2] Univ Chester, Dept Chem Engn, Chester CH1 4BJ, England

来源：

JOURNAL OF COLLOID AND INTERFACE SCIENCE | 2024年 / 661卷

基金：

中国国家自然科学基金;

关键词：

Ag nanoparticles; Silk fibroin microspheres; Microneedle system; Bacterial biofilm; Wound healing; ANTIBACTERIAL; SILVER; CHITOSAN; RELEASE;

D O I：

10.1016/j.jcis.2024.01.147

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Most cases of delayed wound healing are associated with bacterial biofilm infections due to high antibiotic resistance. To improve patient compliance and recovery rates, it is critical to develop minimally invasive and efficient methods to eliminate bacterial biofilms as an alternative to clinical debridement techniques. Herein, we develop a dissolving microneedle system containing Ag nanoparticles (AgNPs)-decorated silk fibroin microspheres (SFM-AgNPs) and antibiotics for synergistic treatment of bacterial biofilm infection. Silk fibroin microspheres (SFM) are controllably prepared in an incompatible system formed by a mixture of protein and carbohydrate solutions by using a mild all-aqueous phase method and serve as biological templates for the synthesis of AgNPs. The SFM-AgNPs exert dose- and time-dependent broad-spectrum antibacterial effects by inducing bacterial adhesion. The combination of SFM-AgNPs with antibiotics breaks the limitation of the antibacterial spectrum and achieves better efficacy with reduced antibiotic dosage. Using hyaluronic acid (HA) as the soluble matrix, the microneedle system containing SFM-AgNPs and anti-Gram-positive coccus drug (Mupirocin) inserts into the bacterial biofilms with sufficient strength, thereby effectively delivering the antibacterial agents and realizing good antibiofilm effect on Staphylococcus aureus-infected wounds. This work demonstrates the great potential for the development of novel therapeutic systems for eradicating bacterial biofilm infections.

引用

页码：123 / 138

页数：16