Identification and investigation of barley powdery mildew (Blumeria graminis f. sp. tritici) infection in winter wheat with conventional stress reactions and non-invasive biophoton emission parameters

被引:4
作者
Jocsak, Ildiko [1 ]
Lukacs, Helga [1 ]
Varga-Visi, Eva [2 ]
Somfalvi-Toth, Katalin [1 ]
Keszthelyi, Sandor [1 ]
机构
[1] Hungarian Univ Agr & Life Sci, Inst Agron, Dept Agron, Kaposvar Campus, Kaposvar, Hungary
[2] Hungarian Univ Agr & Life Sci, Inst Physiol & Nutr, Dept Physiol & Anim Hlth, Kaposvar Campus, H-7400 Kaposvar, Hungary
关键词
Delayed fluorescence; non-invasive stress detection; polymerase chain reaction; powdery mildew; ultra-weak luminescence; wheat; DELAYED FLUORESCENCE; PROMPT FLUORESCENCE; ULTRAWEAK LIGHT; SALT STRESS; LEAVES;
D O I
10.1007/s12038-023-00391-x
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The objective of this study was to characterize the effects of barley powdery mildew infection on wheat via the evolution and dynamics of chloroplasts and oxidative processes based on in vivo measurements of ultra-weak photon emission, parallel measurement of chlorophyll and ascorbic acid content, and molecular identification of the pathogen. The results showed the temporal dynamics of the evolution of ultra-weak photon emission signals that were evidently different for healthy and powdery mildew-infested wheat leaves. In the dark, the ceasing of delayed fluorescence signal made it possible to visualize the ultra-weak luminescence signal as well. Both delayed fluorescence and ultra-weak luminescence signals were characteristic of stress symptoms induced by powdery mildew that was further strengthened by the changes of chlorophyll and ascorbic acid content as typical stress analytical parameters. The presented data and parameterization enabled the identification of stress induction due to powdery mildew infestation in wheat, which should be investigated in detail in the future for fine-tuning our measurements, even by using other species and increasing the length of the measurement in order to increase its specificity. The changes in R-2 values are suitable for monitoring the changes of plant stress response. The measurement of fluorescence and luminescence leads to a greater comprehension of the underlying photon emission-related processes, both in general and in the case of powdery mildew infestation.
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页数:15
相关论文
共 55 条
[1]   Specificity and levels of nonhost resistance to nonadapted Blumeria graminis forms in barley [J].
Aghnoum, Reza ;
Niks, Rients E. .
NEW PHYTOLOGIST, 2010, 185 (01) :275-284
[2]  
[Anonymous], 2023, R Foundation for Statistical Computing
[3]   Disease-weather relationships for powdery mildew and yellow rust on winter wheat [J].
Beest, D. E. Te ;
Paveley, N. D. ;
Shaw, M. W. ;
van den Bosch, F. .
PHYTOPATHOLOGY, 2008, 98 (05) :609-617
[4]   The ferric reducing ability of plasma (FRAP) as a measure of ''antioxidant power'': The FRAP assay [J].
Benzie, IFF ;
Strain, JJ .
ANALYTICAL BIOCHEMISTRY, 1996, 239 (01) :70-76
[5]  
Bodemer U., 2000, Advances in Limnology, V55, P87
[6]   Vitamins for enhancing plant resistance [J].
Boubakri, Hatem ;
Gargouri, Mahmoud ;
Mliki, Ahmed ;
Brini, Faical ;
Chong, Julie ;
Jbara, Moez .
PLANTA, 2016, 244 (03) :529-543
[7]  
Cao XueRen Cao XueRen, 2009, Acta Phytopathologica Sinica, V39, P290
[8]   Effects of Different Planting Densities on Photosynthesis in Maize Determined via Prompt Fluorescence, Delayed Fluorescence and P700 Signals [J].
Chen, Wanying ;
Jia, Bo ;
Chen, Junyu ;
Feng, Yujiao ;
Li, Yue ;
Chen, Miantai ;
Liu, Huanhuan ;
Yin, Zhitong .
PLANTS-BASEL, 2021, 10 (02) :1-17
[9]   Imaging of ultra-weak bio-chemiluminescence and singlet oxygen generation in germinating soybean in response to wounding [J].
Chen, WL ;
Xing, D ;
Tan, SC ;
Tang, YH ;
He, YH .
LUMINESCENCE, 2003, 18 (01) :37-41
[10]   Ultra-weak photon emission from biological samples: Definition, mechanisms, properties, detection and applications [J].
Cifra, Michal ;
Pospisil, Pavel .
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2014, 139 :2-10