Volatile linalool activates grapevine resistance against downy mildew with changes in the leaf metabolome

被引:8
作者
Avesani, Sara [1 ,2 ,3 ]
Lazazzara, Valentina [3 ]
Robatscher, Peter [2 ]
Oberhuber, Michael [2 ]
Perazzolli, Michele [1 ,3 ]
机构
[1] Univ Trento, Ctr Agr Food Environm C3A, Via E Mach 1, I-38089 San Michele All Adige, Italy
[2] Laimburg Res Ctr, Lab Flavours & Metabolites, Laimburg 6, Pfatten Vadena, I-39040 Auer, Italy
[3] Fdn Edmund Mach, Res & Innovat Ctr, Via E Mach 1, I-38098 San Michele All Adige, Italy
关键词
Volatile organic compounds; Induced systemic resistance; Vitis vinifera; Downy mildew; Untargeted metabolomics analysis; Gene expression; TRICHODERMA-HARZIANUM T39; PLASMOPARA-VITICOLA; CONFERS RESISTANCE; DEFENSE RESPONSES; ORGANIC-COMPOUNDS; BACTERIAL-BLIGHT; PLANT VOLATILES; BIOSYNTHESIS; FLOWERS; METABOANALYST;
D O I
10.1016/j.cpb.2023.100298
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Volatile organic compounds (VOCs) are produced by plants in response to biotic and abiotic stimuli. In grapevine, volatile terpenoids are triggered by downy mildew infection (caused by Plasmopara viticola), suggesting their involvement in plant defense responses. In particular, linalool was detected in leaves of downy mildewresistant genotypes, but no information is available on its involvement in the defense mechanisms against P. viticola. The aim of this study was to investigate the defense mechanisms activated by linalool in grapevine leaves against P. viticola and to identify metabolic changes associated with linalool-induced resistance. Linalool treatment reduced downy mildew severity on leaf disks of susceptible grapevines (cultivar Pinot noir) and stimulated callose deposition at the sites of P. viticola infection. Moreover, the upregulation of defense-related genes was found in linalool-treated leaf disks, indicating the activation of grapevine defense mechanisms of salicylic acid and jasmonic acid pathways. Linalool treatment caused changes in the leaf metabolome of mockinoculated and P. viticola-inoculated samples at one and six days post inoculation, as revealed by ultra-high pressure liquid chromatography-electrospray ionization-high-resolution quadrupole time of flight-mass spectrometry. Pathway analysis of annotated features with significant increases and decreases in abundance revealed the reprogramming of amino acid, phenylpropanoid, and terpenoid metabolisms in response to linalool treatment and P. viticola inoculation. In particular, features with significant increases in abundance in linalool-treated samples mainly belonged to putative phenylpropanoids, putative terpenoids, putative lipids, and lipid-like compounds, including molecules possibly associated with plant defense against pathogens, such as 2-phenylethanol, 2,4-heptadienal, alpha-terpineol, citral, and geraniol. These results demonstrated that linalool induces grapevine resistance against downy mildew, acting as a signaling molecule for plant resistance induction.
引用
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页数:11
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共 96 条
[51]   Fungicidal activity of plant volatile compounds for controlling Monilinia laxa in stone fruit [J].
Neri, Fiorella ;
Mari, Marta ;
Brigati, Stefano ;
Bertolini, Paolo .
PLANT DISEASE, 2007, 91 (01) :30-35
[52]   KEGG: Kyoto Encyclopedia of Genes and Genomes [J].
Ogata, H ;
Goto, S ;
Sato, K ;
Fujibuchi, W ;
Bono, H ;
Kanehisa, M .
NUCLEIC ACIDS RESEARCH, 1999, 27 (01) :29-34
[53]   Application of UPLC-QTOF-MS Based Untargeted Metabolomics in Identification of Metabolites Induced in Pathogen-Infected Rice [J].
Oh, Mira ;
Park, SeonJu ;
Kim, Hun ;
Choi, Gyung Ja ;
Kim, Seung Hyun .
PLANTS-BASEL, 2021, 10 (02) :1-13
[54]   3¶Untargeted metabolomic analysis by ultra-high-resolution mass spectrometry for the profiling of new Italian wine varieties [J].
Onzo, Alberto ;
Acquavia, Maria Assunta ;
Pascale, Raffaella ;
Iannece, Patrizia ;
Gaeta, Carmine ;
Lelario, Filomena ;
Ciriello, Rosanna ;
Tesoro, Carmen ;
Bianco, Giuliana ;
Di Capua, Angela .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2022, 414 (27) :7805-7812
[55]   Proteomic analysis of grapevine resistance induced by Trichoderma harzianum T39 reveals specific defence pathways activated against downy mildew [J].
Palmieri, Maria Cristina ;
Perazzolli, Michele ;
Matafora, Vittoria ;
Moretto, Marco ;
Bachi, Angela ;
Pertot, Ilaria .
JOURNAL OF EXPERIMENTAL BOTANY, 2012, 63 (17) :6237-6251
[56]   MetaboAnalyst 5.0: narrowing the gap between raw spectra and functional insights [J].
Pang, Zhiqiang ;
Chong, Jasmine ;
Zhou, Guangyan ;
Morais, David Anderson de Lima ;
Chang, Le ;
Barrette, Michel ;
Gauthier, Carol ;
Jacques, Pierre-Etienne ;
Li, Shuzhao ;
Xia, Jianguo .
NUCLEIC ACIDS RESEARCH, 2021, 49 (W1) :W388-W396
[57]   Downy mildew resistance induced by Trichoderma harzianum T39 in susceptible grapevines partially mimics transcriptional changes of resistant genotypes [J].
Perazzolli, Michele ;
Moretto, Marco ;
Fontana, Paolo ;
Ferrarini, Alberto ;
Velasco, Riccardo ;
Moser, Claudio ;
Delledonne, Massimo ;
Pertot, Ilaria .
BMC GENOMICS, 2012, 13
[58]   Glycosylation and oxidative dimerization of resveratrol are respectively associated to sensitivity and resistance of grapevine cultivars to downy mildew [J].
Pezet, R ;
Gindro, K ;
Viret, O ;
Spring, JL .
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 2004, 65 (06) :297-303
[59]   Ecology of plant volatiles: taking a plant community perspective [J].
Pierik, Ronald ;
Ballare, Carlos L. ;
Dicke, Marcel .
PLANT CELL AND ENVIRONMENT, 2014, 37 (08) :1845-1853
[60]   A Non-targeted Metabolomics Approach Unravels the VOCs Associated with the Tomato Immune Response against Pseudomonas syringae [J].
Pilar Lopez-Gresa, Maria ;
Lison, Purificacion ;
Campos, Laura ;
Rodrigo, Ismael ;
Luis Rambla, Jose ;
Granell, Antonio ;
Conejero, Vicente ;
Maria Belles, Jose .
FRONTIERS IN PLANT SCIENCE, 2017, 8