Evaluation of Toxicity Equivalency Factors of Tetrodotoxin Analogues with a Neuro-2a Cell-Based Assay and Application to Puffer Fish from Greece

被引：10

作者：

Alkassar, Mounira ^{[1
]}

Sanchez-Henao, Andres ^{[1
]}

Reverte, Jaume ^{[1
]}

Barreiro, Lourdes ^{[1
]}

Rambla-Alegre, Maria ^{[1
]}

Leonardo, Sandra ^{[1
]}

Mandalakis, Manolis ^{[2
]}

Peristeraki, Panagiota ^{[3
]}

Diogene, Jorge ^{[1
]}

Campas, Monica ^{[1
]}

机构：

[1] Inst Recerca & Tecnol Agroalimentaries IRTA, Ctra Poble Nou Km 5-5, La Rapita 43540, Spain

[2] Hellen Ctr Marine Res, Inst Marine Biol Biotechnol & Aquaculture, Iraklion 71003, Greece

[3] Hellen Ctr Marine Res, Inst Marine Biol Resources & Inland Waters, Iraklion 71003, Greece

来源：

MARINE DRUGS | 2023年 / 21卷 / 08期

关键词：

tetrodotoxin (TTX); TTX analogue; toxicity equivalency factor (TEF); cell-based assay (CBA); liquid chromatography-tandem mass spectrometry (LC-MS/MS); puffer fish; Lagocephalus sceleratus; LAGOCEPHALUS-SCELERATUS GMELIN; MASS-SPECTROMETRY METHOD; TISSUE-CULTURE BIOASSAY; NEUROBLASTOMA-CELLS; SODIUM-CHANNEL; 1ST DETECTION; SAXITOXIN; CAUGHT; TOXINS; TTX;

D O I：

10.3390/md21080432

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Tetrodotoxin (TTX) is a potent marine neurotoxin involved in poisoning cases, especially through the consumption of puffer fish. Knowledge of the toxicity equivalency factors (TEFs) of TTX analogues is crucial in monitoring programs to estimate the toxicity of samples analyzed with instrumental analysis methods. In this work, TTX analogues were isolated from the liver of a Lagocephalus sceleratus individual caught on South Crete coasts. A cell-based assay (CBA) for TTXs was optimized and applied to the establishment of the TEFs of 5,11-dideoxyTTX, 11-norTTX-6(S)-ol, 11-deoxyTTX and 5,6,11-trideoxyTTX. Results showed that all TTX analogues were less toxic than the parent TTX, their TEFs being in the range of 0.75-0.011. Then, different tissues of three Lagocephalus sceleratus individuals were analyzed with CBA and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The obtained TEFs were applied to the TTX analogues' concentrations obtained by LC-MS/MS analysis, providing an indication of the overall toxicity of the sample. Information about the TEFs of TTX analogues is valuable for food safety control, allowing the estimation of the risk of fish products to consumers.

引用

页数：22

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