Characteristics of rare ST463 carbapenem-resistant Pseudomonas aeruginosa clinical isolates from blood

被引:4
|
作者
Zhang, Xiaodong [1 ]
Tang, Miran [1 ]
Xu, Ye [2 ]
Xu, Mengxin [1 ]
Qian, Changrui [3 ]
Zheng, Xiangkuo [1 ]
Zhou, Tieli [1 ,4 ]
Wu, Qing [1 ,4 ]
机构
[1] Wenzhou Med Univ, Dept Clin Lab, Key Lab Clin Lab Diag & Translat Res Zhejiang Prov, Affiliated Hosp 1, Wenzhou, Peoples R China
[2] Southern Med Univ, Affiliated Hosp 5, Dept Clin Lab, Guangzhou, Peoples R China
[3] Wenzhou Med Univ, Sch Basic Med Sci, Wenzhou, Peoples R China
[4] Wenzhou Med Univ, Dept Clin, Key Lab Clin Lab Diag & Translat Res Zhejiang Prov, Affiliated Hosp 1, Wenzhou 325000, Peoples R China
关键词
bla KPC-2; bla OXA-486; Carbapenem-resistant Pseudomonas  aeruginosa; crpP; ST463; Virulence;
D O I
10.1016/j.jgar.2023.01.011
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Objectives: This study aimed to elucidate resistance to carbapenems and fluoroquinolones, the transmis-sion mechanism of blaKPC-2, and the virulence characteristics of a Pseudomonas aeruginosa strain (TL3773) isolated in East China.Methods: The virulence and resistance mechanisms of TL3773 were investigated by whole genome se-quencing (WGS), comparative genomic analysis, conjugation experiments, and virulence assays. Results: This study isolated carbapenem-resistant P. aeruginosa from blood resistant to carbapen-ems. The patient's clinical data showed poor prognosis compounded by multiple sites of infec-tion. WGS showed that TL3773 carried aph (3')-IIb, blaPAO, bla OXA-486, fosA, catB7, and two crpP re-sistance genes on chromosome, and the carbapenem resistance gene blaKPC-2 on plasmid. We iden-tified a novel crpP gene named TL3773-crpP2. Cloning experiments proved that TL3773-crpP2 was not the primary cause of fluoroquinolone resistance in TL3773. GyrA and ParC mutations may con-fer fluoroquinolone resistance. The blaKPC-2 genetic environment was IS26-TnpR-ISKpn27- blaKPC-2-ISKpn6- IS26-Tn3-IS26, potentially mediating the transmission of blaKPC-2 in P. aeruginosa. The overall vir-ulence of TL3773 was lower than that of PAO1. However, the pyocyanin and biofilm formation of TL3773 was higher than that of PAO1. WGS further indicated that TL3773 was less virulent than PAO1. Phylogenetic analysis showed that TL3773 was most similar to the P. aeruginosa isolate ZYPA29 from Hangzhou, China. These observations further indicate that ST463 P. aeruginosa is rapidly spreading.Conclusions: The threat of ST463 P. aeruginosa harbouring blaKPC-2 is emergent and may pose a threat to human health. More extensive surveillance and effective actions are urgently needed to control its further spread.(c) 2023 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )
引用
收藏
页码:122 / 130
页数:9
相关论文
共 50 条
  • [31] Clinical Characteristics and Prognosis of Bloodstream Infection with Carbapenem-Resistant Pseudomonas aeruginosa in Patients with Hematologic Malignancies
    Yuan, Fangfang
    Xiao, Weiqiang
    Wang, Xiaokun
    Fu, Yuewen
    Wei, Xudong
    INFECTION AND DRUG RESISTANCE, 2023, 16 : 4943 - 4952
  • [32] Epidemiological and Genetic Characteristics of Clinical Carbapenem-Resistant Pseudomonas aeruginosa Strains in Guangdong Province, China
    Zhao, Yonggang
    Chen, Dingqiang
    Chen, Kaichao
    Xie, Miaomiao
    Guo, Jiubiao
    Chan, Edward Wai Chi
    Xie, Lu
    Wang, Jingbo
    Chen, Enqi
    Chen, Sheng
    Chen, Weijun
    Jelsbak, Lars
    MICROBIOLOGY SPECTRUM, 2023, 11 (03):
  • [33] Prevalence of class I, II and III integrons in multidrug-resistant and carbapenem-resistant Pseudomonas aeruginosa clinical isolates
    Khademi, Farzad
    Ashrafi, Shahryar Seyed
    Neyestani, Zohreh
    Vaez, Hamid
    Sahebkar, Amirhossein
    GENE REPORTS, 2021, 25
  • [34] Characteristics of carbapenem-resistant Enterobacteriaceae isolates from Korea
    Kim, So Yeon
    Shin, Juyoun
    Shin, Sang Yop
    Ko, Kwan Soo
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2013, 76 (04) : 486 - 490
  • [35] Dissemination of carbapenem-resistant Pseudomonas aeruginosa isolates and their susceptibilities to ceftolozane-tazobactam in Germany
    Kresken, Michael
    Koerber-Irrgang, Barbara
    Korte-Berwanger, Miriam
    Pfennigwerth, Niels
    Gatermann, Soeren G.
    Seifert, Harald
    INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2020, 55 (06)
  • [36] Distribution of Pseudomonas-Derived Cephalosporinase and Metallo-β-Ladamases in Carbapenem-Resistant Pseudomonas aeruginosa Isolates from Korea
    Choi, Hye Hyun
    Kwon, Gye Cheol
    Kim, Semi
    Koo, Sun Hoe
    JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2015, 25 (07) : 1154 - 1162
  • [37] An outbreak of carbapenem-resistant Pseudomonas aeruginosa in a urology ward
    Peña, C
    Dominguez, MA
    Pujol, M
    Verdaguer, R
    Gudiol, F
    Ariza, J
    CLINICAL MICROBIOLOGY AND INFECTION, 2003, 9 (09) : 938 - 943
  • [38] Selection of carbapenem-resistant Pseudomonas aeruginosa in a haematology unit?
    Garvey, M. I.
    Bradley, C. W.
    Biggs, M. J.
    Holden, E.
    Gill, M. J.
    JOURNAL OF HOSPITAL INFECTION, 2018, 98 (03) : 238 - 240
  • [39] Carbapenem-resistant Pseudomonas aeruginosa and carbapenem use in Japan: an ecological study
    Terahara, Fumitaka
    Nishiura, Hiroshi
    JOURNAL OF INTERNATIONAL MEDICAL RESEARCH, 2019, 47 (10) : 4711 - 4722
  • [40] The epidemiology and virulence of carbapenem-resistant Pseudomonas aeruginosa in China
    Zhu, Ying
    Jia, Peiyao
    Yu, Wei
    Chu, Xiaobing
    Liu, Xiaoyu
    Yang, Qiwen
    LANCET MICROBE, 2023, 4 (09): : E665 - E665