Retrograde peripheral nerve regeneration from sensory to motor pathways in rats: a new experimental concept in nerve repair

被引:2
作者
Bertelli, Jayme A. [1 ,4 ]
Rosa, Isadora Carvalho Nunes [2 ]
Ghizoni, Marcos F. [3 ]
机构
[1] Univ Fed Santa Catarina, Dept Surg lieu, Dept Surg Tech, Florianopolis, SC, Brazil
[2] Univ South St Catarina UNISUL, Ctr Biol & Hlth Sci, Tubarao, SC, Brazil
[3] Univ South Santa Catarina UNISUL, Ctr Biol & Hlth Sci, Dept Neurosurg, Tubarao, SC, Brazil
[4] Univ Fed Santa Catarina, Dept Surg, Rua Eng Agron Andrei Cristian Ferreira S-N, BR-88040900 Florianopolis, SC, Brazil
关键词
Nerve graft; retrograde nerve regeneration; nerve transfer; muscle reinnervation; nerve regeneration; nerve repair; BRACHIAL-PLEXUS; FUNCTIONAL RECOVERY; BRANCH TRANSFER; BASAL LAMINA; INJURY; SPECIFICITY; TRANSFERS; FORELIMB; MODEL;
D O I
10.1080/01616412.2023.2258039
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
BackgroundThe polarity of nerve grafts does not interfere with axon growth. Our goal was to investigate whether axons can regenerate in a retrograde fashion within sensory pathways and then extend into motor pathways, leading to muscle reinnervation.MethodsFifty-four rats were randomized into four groups. In Group 1, the ulnar nerve was connected end-to-end to the superficial radial nerve after neurectomy of the radial nerve in the axilla. In Group 2, the ulnar nerve was connected end-to-end to the radial nerve distal to the humerus; the radial nerve then was divided in the axilla. In Group 3, the radial nerve was divided in the axilla, but no nerve reconstruction was performed. In Group 4, the radial nerve was crushed in the axilla. Over 6 months, we behaviorally assessed the recovery of toe spread in the right operated-upon forepaw by lifting the rat by its tail and lowering it onto a flat surface. Six months after surgery, rats underwent reoperation, nerve transfers were tested electrophysiologically, and the posterior interosseous nerve (PIN) was removed for histological evaluation.ResultsRats in the crush group recovered toe spread between 5 and 8 days after surgery. Rats with nerve transfers demonstrated electrophysiological and histological findings of nerve regeneration but no behavioral recovery.ConclusionsUlnar nerve axons regrew into the superficial radial nerve and then into the PIN to reinnervate the extensor digitorum communis. We were unable to demonstrate behavioral recovery because rats cannot readapt to cross-nerve transfer.
引用
收藏
页码:125 / 131
页数:7
相关论文
共 48 条
[1]   Specificity of peripheral nerve regeneration: Interactions at the axon level [J].
Allodi, Ilary ;
Udina, Esther ;
Navarro, Xavier .
PROGRESS IN NEUROBIOLOGY, 2012, 98 (01) :16-37
[2]   Elbow flexion reconstruction with nerve transfer or grafting in patients with brachial plexus injuries: A systematic review and comparison study [J].
Ayhan, Egemen ;
Soldado, Francisco ;
Fontecha, Cesar G. ;
Bertelli, Jayme A. ;
Leblebicioglu, Gursel .
MICROSURGERY, 2020, 40 (01) :79-86
[3]   RAT BRACHIAL-PLEXUS AND ITS TERMINAL BRANCHES - AN EXPERIMENTAL-MODEL FOR THE STUDY OF PERIPHERAL-NERVE REGENERATION [J].
BERTELLI, JA ;
TALEB, M ;
SAADI, A ;
MIRA, JC ;
PECOTDECHAVASSINE, M .
MICROSURGERY, 1995, 16 (02) :77-85
[4]   THE GRASPING TEST - A SIMPLE BEHAVIORAL-METHOD FOR OBJECTIVE QUANTITATIVE ASSESSMENT OF PERIPHERAL-NERVE REGENERATION IN THE RAT [J].
BERTELLI, JA ;
MIRA, JC .
JOURNAL OF NEUROSCIENCE METHODS, 1995, 58 (1-2) :151-155
[5]   Outcomes of Radial Nerve Grafting In Children After Distal Humerus Fracture [J].
Bertelli, Jayme ;
Soldado, Francisco ;
Ghizoni, Marcos F. .
JOURNAL OF HAND SURGERY-AMERICAN VOLUME, 2018, 43 (12) :1140.e1-1140.e6
[6]   Concepts of nerve regeneration and repair applied to brachial plexus reconstruction [J].
Bertelli, Jayme Augusto ;
Ghizoni, Marcos Flavio .
MICROSURGERY, 2006, 26 (04) :230-244
[7]   EXPERIMENTAL INTERFASCICULAR NERVE GRAFTING [J].
BRATTON, BR ;
KLINE, DG ;
COLEMAN, W ;
HUDSON, AR .
JOURNAL OF NEUROSURGERY, 1979, 51 (03) :323-332
[8]  
Brown Justin M, 2009, Neurosurg Focus, V26, pE12, DOI 10.3171/FOC.2009.26.2.E12
[9]  
Chin TY, 2017, MALAYS J MED SCI, V24, P31, DOI 10.21315/mjms2017.24.1.4
[10]   Role of Inflammation and Cytokines in Peripheral Nerve Regeneration [J].
Dubovy, Petr ;
Jancalek, Radim ;
Kubek, Tomas .
TISSUE ENGINEERING OF THE PERIPHERAL NERVE: STEM CELLS AND REGENERATION PROMOTING FACTORS, 2013, 108 :173-206