Thiopental sodium attenuates hypoxia/reoxygenation-induced injury in osteoblasts by modulating AKT signaling

被引:1
作者
Hu, Chuan-yu [1 ]
Li, Guo-yan [2 ]
Li, Chun-Tian [3 ]
机构
[1] Beijing Univ Chinese Med, Dept Orthoped, Dongzhimen Hosp, Ward 4, Beijing 100700, Peoples R China
[2] Beijing Univ Chinese Med, Dept Anesthesiol, Dongzhimen Hosp, Beijing 100700, Peoples R China
[3] Beijing Univ Chinese Med, Dept Acupuncture, Dongzhimen Hosp, Beijing 100700, Peoples R China
关键词
Thiopental sodium; Hypoxia; reoxygenation; reoxygenation-induced injury; Osteoblasts; AKT; ISCHEMIA-REPERFUSION INJURY; ISCHEMIA/REPERFUSION INJURY; IN-VITRO; PROTECTS; PATHOPHYSIOLOGY; APOPTOSIS;
D O I
10.1007/s11626-023-00801-2
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Thiopental sodium (TPTS) is a barbiturate general anesthetic, while its effects on hypoxia/reoxygenation (H/R)-induced injury are still unclear. This study aimed to investigate whether TPTS exerts protective effects against the H/R-induced osteoblast cell injury and explore the underlying mechanisms. Osteoblast cell injury model was induced by the H/R condition, which was treated with or without TPTS. Cell viability and lactate dehydrogenase (LDH) release were determined by the corresponding commercial kits. The levels of oxidative stress were determined in the experimental groups. Cell apoptosis and Caspase-3 activities were determined by propidium iodide staining and substrate-based assay, respectively. Western blotting and qRT-PCR were performed to measure the mRNA and protein levels, respectively. Treatment with TPTS was able to increase cell viability and reduce LDH release in H/R-induced osteoblasts. Additionally, TPTS regulated oxidative stress in H/R-induced osteoblasts by suppressing malondialdehyde (MDA) and reactive oxygen species (ROS) as well as boosting superoxide dismutase (SOD). TPTS was able to suppress cell apoptosis by suppressing Caspase-3 activity and cleavage. TPTS exerted protective effects against cell injury and apoptosis induced by the H/R conditions, which were associated with its regulation of Akt signaling. Moreover, TPTS induced osteoblast differentiation under the H/R condition. In summary, TPTS attenuates H/R-induced injury in osteoblasts by regulating AKT signaling.
引用
收藏
页码:528 / 535
页数:8
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