It takes two to tango: cardiac fibroblast-derived NO-induced cGMP enters cardiac myocytes and increases cAMP by inhibiting PDE3

被引:3
作者
Menges, Lukas [1 ]
Giesen, Jan [1 ]
Yilmaz, Kerem [1 ]
Mergia, Evanthia [1 ]
Fuechtbauer, Annette [2 ]
Fuechtbauer, Ernst-Martin [2 ]
Koesling, Doris [1 ]
Russwurm, Michael [1 ]
机构
[1] Ruhr Univ Bochum, Inst Pharmacol & Toxicol, D-44780 Bochum, Germany
[2] Aarhus Univ, Dept Mol Biol & Genet, DK-8000 Aarhus C, Denmark
关键词
NITRIC-OXIDE SYNTHASE; CYCLIC-GMP; CYCLASE; HEART; PHOSPHODIESTERASES; NITROVASODILATORS; RECOMBINATION; CONTRACTILITY; SENSITIVITY; RELAXATION;
D O I
10.1038/s42003-023-04880-5
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The occurrence of NO/cGMP signalling in cardiac cells is a matter of debate. Recent measurements with a FRET-based cGMP indicator in isolated cardiac cells revealed NO-induced cGMP signals in cardiac fibroblasts while cardiomyocytes were devoid of these signals. In a fibroblast/myocyte co-culture model though, cGMP formed in fibroblasts in response to NO entered cardiomyocytes via gap junctions. Here, we demonstrate gap junction-mediated cGMP transfer from cardiac fibroblasts to myocytes in intact tissue. In living cardiac slices of mice with cardiomyocyte-specific expression of a FRET-based cGMP indicator (alpha MHC/cGi-500), NO-dependent cGMP signals were shown to occur in myocytes, to depend on gap junctions and to be degraded mainly by PDE3. Stimulation of NO-sensitive guanylyl cyclase enhanced Forskolin- and Isoproterenol-induced cAMP and phospholamban phosphorylation. Genetic inactivation of NO-GC in Tcf21-expressing cardiac fibroblasts abrogated the synergistic action of NO-GC stimulation on Iso-induced phospholamban phosphorylation, identifying fibroblasts as cGMP source and substantiating the necessity of cGMP-transfer to myocytes. In sum, NO-stimulated cGMP formed in cardiac fibroblasts enters cardiomyocytes in native tissue where it exerts an inhibitory effect on cAMP degradation by PDE3, thereby increasing cAMP and downstream effects in cardiomyocytes. Hence, enhancing beta-receptor-induced contractile responses appears as one of NO/cGMP's functions in the non-failing heart. The use of living cardiac slices of mice demonstrates the occurrence of NO- and gap junction-dependent cGMP in myocytes increasing isoproterenol-induced cAMP and phospholamban phosphorylation, with fibroblasts as NO-dependent cGMP source.
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页数:12
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